Clove Bud Oil Modulates Pathogenicity Phenotypes of the Opportunistic Human Pathogen Pseudomonas aeruginosa
Autor: | Jayalekshmi Haripriyan, Muralidharan Vanuopadath, Athira Omanakuttan, Nitasha D. Menon, Bipin G. Nair, Sudarslal Sadasivan Nair, Victor Nizet, Geetha B. Kumar, Ross Corriden |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Proteases Virulence Factors 030106 microbiology lcsh:Medicine Virulence Human pathogen Biology medicine.disease_cause Extracellular Traps Article Microbiology Mice 03 medical and health sciences Bacterial Proteins 3T3-L1 Cells Endopeptidases medicine Animals Humans Pseudomonas Infections Enzyme Inhibitors Caenorhabditis elegans lcsh:Science Pathogen Multidisciplinary Pancreatic Elastase Pseudomonas aeruginosa lcsh:R Elastase Quorum Sensing Neutrophil extracellular traps Anti-Bacterial Agents Quorum sensing Clove Oil Matrix Metalloproteinase 2 lcsh:Q Peptide Hydrolases |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Earlier studies from our laboratory have demonstrated that clove bud oil (CBO) attenuates expression of certain virulence factors of Pseudomonas aeruginosa PAO1. Here, we probe more deeply into the effect of CBO on four pseudomonal proteases - elastase A, elastase B, protease IV and alkaline protease - each known to play key roles in disease pathogenesis. CBO inhibited the activity of these proteases present in the bacterial culture supernatant. Zymography studies indicated that these proteases can activate host matrix metalloproteases (MMPs) to establish infection, through conversion of pro-MMP-2 to active MMP-2. PAO1 is a predominant pathogen in burn wound infections and we show the modulatory effect of CBO on MMPs in an in vitro model of burn injury. Furthermore, CBO induced dose-dependent neutrophil extracellular trap formation in human neutrophils. CBO also increased the survival of C. elegans infected with PAO1, establishing an anti-infective role in a whole animal model of pathogenesis. LC-MS/MS analysis indicated that CBO treatment elicited a significant reduction of signalling molecules (Acyl-Homoserine-Lactone) involved in quorum sensing regulation. Our observations demonstrate that CBO attenuates key virulence mechanisms of this important human pathogen, while concomitantly enhancing host innate immunomodulatory functions, with potential implications for topical therapy against antibiotic-resistant infections. |
Databáze: | OpenAIRE |
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