Nonrandom loss of human chromosome 3 fragments from mouse-human microcell hybrids following progressive growth in SCID mice
| Autor: | Irina Kholodnyuk, Rando Allikmetts, Eugene R. Zabarovsky, Eric J. Stanbridge, Stephan Imreh, George Klein |
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| Rok vydání: | 1994 |
| Předmět: |
Genetic Markers
Cancer Research Fibrosarcoma Molecular Sequence Data Cell Mice SCID In situ hybridization Hybrid Cells Biology Polymerase Chain Reaction law.invention Mice In vivo law Genetics medicine Animals Humans Genes Tumor Suppressor Gene In Situ Hybridization Fluorescence Base Sequence Chromosome Molecular biology medicine.anatomical_structure Chromosome 3 Cell culture Karyotyping Suppressor Chromosomes Human Pair 3 Chromosome Deletion Cell Division |
| Zdroj: | Genes, Chromosomes and Cancer. 11:237-245 |
| ISSN: | 1098-2264 1045-2257 |
| DOI: | 10.1002/gcc.2870110406 |
| Popis: | Microcell hybrid lines of A9 mouse fibrosarcoma containing complete or partially deleted human chromosomes 3 (chr. 3) were inoculated into SCID mice. Cell lines derived from the tumors were examined by fluorescent in situ hybridization for the status of the transferred human chromosome and by PCR for marker loss. The SCID tumors arising after the inoculation of 10(5) cells were passaged serially in vivo and regularly showed loss of four markers; D3S1029 (3p21.3-21.2), AP20R (3p22-21.3, D3S32 (3p21.3-p21.2), and THRB (3p24). This regularly deleted region is bordered by markers GNA12 (3p21.1-p21.3) and VHL (3p25) that were maintained in a fraction of tumors. Fragments derived from the long arm of chromosome 3 and corresponding markers in the 3q26-q28 region were retained in all tumors. Our findings may be related to the postulated presence of tumor suppressor genes in the 3p24-p21 region as indicated by the frequent deletion of this region in renal and small cell lung carcinomas and other solid tumors. The technically cumbersome identification of suppressor genes may be supplemented by an "elimination test" based on analogous principles. |
| Databáze: | OpenAIRE |
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