Differential requirements for the Ets transcription factor Elf-1 in the development of NKT cells and NK cells
| Autor: | Kyrie Felio, Pramod K. Giri, Yanbiao Geng, Sha Li, Hak Jong Choi, Chyung Ru Wang, Hoonsik Cho |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Myeloid
Cell Survival medicine.medical_treatment Immunology Antigen presentation CD1 chemical and pharmacologic phenomena Biology In Vitro Techniques Biochemistry Proto-Oncogene Protein c-ets-1 Mice Mice Congenic Antigen medicine Animals Transcription factor Immunobiology Mice Knockout Antigen Presentation Transplantation Chimera ETS transcription factor family Nuclear Proteins Cell Differentiation Cell Biology Hematology Natural killer T cell Cell biology DNA-Binding Proteins Killer Cells Natural Mice Inbred C57BL medicine.anatomical_structure Cytokine Cytokines Natural Killer T-Cells Antigens CD1d Genes T-Cell Receptor alpha Transcription Factors |
| Zdroj: | Blood. 117(6) |
| ISSN: | 1528-0020 |
| Popis: | E26 Transformation specific (Ets) family transcription factors control the expression of a large number of genes regulating hematopoietic cell development and function. Two such transcription factors, Ets-1 and myeloid Elf-1–like factor (MEF), have been shown to play critical roles in both natural killer (NK)– and NKT-cell development, but not in the development of conventional T cells. In this study, we address the role of E74-like factor 1 (Elf-1), another Ets family transcription factor that is closely related to MEF but divergent from Ets-1, in NK- and NKT-cell development using Elf-1–deficient (Elf-1−/−) mice. Whereas the proportion of NK cells in Elf-1−/− mice was normal, the proportion of NKT cells was significantly reduced in the thymus and periphery of Elf-1−/− mice compared with wild-type (WT) mice. Although Ets-1–deficient mice lack NKT cells altogether, Elf-1−/− mice exhibited only a partial block in NKT-cell development caused by a cell-intrinsic defect in the selection, survival, and maturation of NKT cells. In addition, residual NKT cells found in Elf-1−/− mice produced less cytokine upon antigen stimulation compared with WT NKT cells. Our data demonstrate that Elf-1 plays an important and nonredundant role in the development and function of NKT cells, but is not involved in NK-cell development. |
| Databáze: | OpenAIRE |
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