Isolation of differentially expressed sex genes in garden asparagus using suppression subtractive hybridization
| Autor: | Tian-yu Dong, Long-Dou Lu, Shao-jing Wang, Shu-Fen Li, Chuan-Liang Deng, Ning-Na Wang, Xin-peng Zhao, Wu-Jun Gao |
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| Rok vydání: | 2014 |
| Předmět: |
Subtractive Hybridization Techniques
DNA Complementary Sequence analysis Molecular Sequence Data Plant Science Flowers Genes Plant Real-Time Polymerase Chain Reaction Gene Expression Regulation Plant Botany Asparagus Gene Gene Library Plant Proteins Genetics Expressed Sequence Tags Expressed sequence tag biology Subtraction hybridization fungi food and beverages Sequence Analysis DNA biology.organism_classification Sexual dimorphism Suppression subtractive hybridization Asparagus Plant |
| Zdroj: | Journal of plant research. 128(5) |
| ISSN: | 1618-0860 |
| Popis: | Garden asparagus (Asparagus officinalis L.) is a dioecious species whose male and female flowers are found in separate unisexual individuals. A region called the M-locus, located on a pair of homomorphic sex chromosomes, controls sexual dimorphism in asparagus. To date, no sex determining gene has been isolated from asparagus. To identify more genes involved in flower development in asparagus, subtractive hybridization library of male flowers in asparagus was constructed by suppression subtraction hybridization. A total of 107 expressed sequence tags (ESTs) were identified. BLASTX analysis showed that the library contained several genes that could be related to flower development. The expression patterns of seven selected genes believed to be involved in the development of asparagus male flower were further analyzed by semi-quantitative or real-time reverse-transcription polymerase chain reaction (RT-PCR). Results showed that AOEST4-5, AOEST12-40, and AOEST13-38 were strongly expressed in the male flower stage, whereas no transcript level of AOEST13-38 was detected in the female flower stage. The expression levels of AOEST13-87, AOEST13-92, AOEST13-40, and AOEST18-87 in the male flower stage were also higher than those in the female flower stage, although these transcripts were also expressed in other tissues. The identified genes can provide a strong starting point for further studies on the underlying molecular differences between the male and female flowers of asparagus. |
| Databáze: | OpenAIRE |
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