Tunable graphdiyne for DNA surface adsorption: affinities, displacement, and applications for fluorescence sensing

Autor:	Xiaofang Li, Haowen Huang, Jinglun Wang, Keqin Deng, Jing Xiao, Chunxiang Li, Zhang Liu, Qingfeng Yi
Rok vydání:	2021
Předmět:	Surface Properties Fluorescence sensing 02 engineering and technology 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Adsorption Low affinity Limit of Detection Fluorescent Dyes Spectrum Analysis Hybridization probe 010401 analytical chemistry DNA 021001 nanoscience & nanotechnology Ochratoxins Fluorescence Affinities 0104 chemical sciences High surface Spectrometry Fluorescence chemistry Microscopy Electron Scanning Biophysics Graphite 0210 nano-technology
Zdroj:	Analytical and Bioanalytical Chemistry. 413:3847-3859
ISSN:	1618-2650 1618-2642
DOI:	10.1007/s00216-021-03355-y
Popis:	Graphdiyne (GDY) adsorbed DNA probes have been used as a fluorescent sensing platform, but topics including DNA adsorption affinities, DNA probe displacement, and fluorescence quenching ability were rarely researched. Herein, the adsorption affinity of single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) on a tremella-like GDY was tuned by modulating the surface chemistry of GDY. The fluorescence quenching ability of GDY with different oxidation degrees was compared. The nonspecific displacement of DNA probes on GDY was studied. Under the same concentrations, GDY with low oxidation degree exhibited stronger adsorption affinity and higher adsorption capacity to both ssDNA and dsDNA than highly oxidized GDY. DNA adsorbed on low-oxidized GDY was more resistant to displacement by other DNAs. Protein showed strong interaction with different GDY and could displace DNA probes on GDY. Based on these findings, an ideal GDY with proper oxidation degree, exhibiting high surface affinity for ssDNA and low affinity for dsDNA, was used as scavenger of redundant ssDNA fluorescent probe in an enzyme-assisted amplification system for sensitive ochratoxin (OTA) detection. This study has enhanced our fundamental understanding of DNA adsorption by GDY. It also provided a rational way to apply GDY for fluorescence sensing in a complicated system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c63983fea5201d55048fd5f8d1a2a399 https://doi.org/10.1007/s00216-021-03355-y Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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