Green synthesis of Pluronic stabilized reduced graphene oxide: Chemical and biological characterization
| Autor: | Susan Sandeman, J. Ashtami, Reshma S Cherian, Irina N. Savina, Santanu Ray, P.V. Mohanan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Reducing agent
Oxide Poloxamer 02 engineering and technology Spectrum Analysis Raman PC12 Cells 01 natural sciences law.invention Mice chemistry.chemical_compound Colloid and Surface Chemistry In vivo law 0103 physical sciences Toxicity Tests Acute Animals Physical and Theoretical Chemistry Cytotoxicity Cytoskeleton Cell Death L-Lactate Dehydrogenase 010304 chemical physics Graphene Photoelectron Spectroscopy In vitro toxicology Green Chemistry Technology Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Ascorbic acid Acute toxicity Rats Endotoxins chemistry Biophysics Graphite Protein Corona Reactive Oxygen Species 0210 nano-technology Oxidation-Reduction Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 179:94-106 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2019.03.043 |
| Popis: | The wonder material graphene has numerous potential applications in nanoelectronics, biomedicine, storage devices, etc. Synthesis of graphene is highly challenging due to the toxic chemicals used and its low yield. In the present study, a facile green route for synthesis of reduced graphene oxide (rGO) was carried out using ascorbic acid as reducing agent. rGO was stabilized using Pluronic P123 polymer to give Pluronic stabilized reduced graphene oxide (rGO-P) and gave superior yield (15 mg graphene oxide yielded ˜13 mg rGO-P). Despite the potential neuroscience applications of graphene, the impending toxicological outcome upon interaction with neurons is not well understood. Here, differentiated PC-12 neuron-like cells exposed to rGO-P showed a dose-dependent cytotoxicity. Membrane disruption and cytoskeletal integrity remained uncompromised after 24 h exposure. Oxidative stress in PC-12 was evident due to an increase in ROS generation in dose and time-dependent manner. In vivo acute toxicity was assessed in mice administered with 10 mg/kg body weight of rGO-P. There were no evident changes in behaviour, motor function or other morphological changes. In conclusion, rGO-P was successfully synthesized and provided superior yield. Even though in vitro toxicity testing showed dose-dependent toxicity, in vivo toxic effect was not apparent. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect