Characterization and cytocompatibility of an antibiotic/chitosan/cyclodextrins nanocoating on titanium implants
| Autor: | Adriana Trapani, Monica Mattioli-Belmonte, Stefania Cometa, Mirella Falconi, Elvira De Giglio, Concetta Ferretti, Edmondo Ceci, Roberta Iatta |
|---|---|
| Přispěvatelé: | Monica Mattioli-Belmonte, Stefania Cometa, Concetta Ferretti, Roberta Iatta, Adriana Trapani, Edmondo Ceci, Mirella Falconi, Elvira De Giglio |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Staphylococcus aureus
Prosthesis-Related Infections Polymers and Plastics chemistry.chemical_element Nanoparticle Beta-Cyclodextrins Orthopaedic infections engineering.material Cell Line Chitosan chemistry.chemical_compound Coated Materials Biocompatible Coating Ciprofloxacin Materials Chemistry Zeta potential medicine Humans Cyclodextrin Titanium beta-Cyclodextrins Organic Chemistry Prostheses and Implants Adhesion Staphylococcal Infections Chitosan nanoparticle Cytocompatibility Anti-Bacterial Agents chemistry engineering Nanoparticles Gene expression gamma-Cyclodextrins medicine.drug Nuclear chemistry |
| Popis: | A novel ciprofloxacin loaded chitosan nanoparticle-based coating onto titanium substrates has been developed and characterized to obtain an orthopaedic implant surface able to in situ release the antibiotic for the prevention of post-operative infections. Ciprofloxacin loaded chitosan nanoparticles were obtained using the combination of sulfobutyl ether-beta-cyclodextrin and gamma-cyclodextrin. The resulting nanoparticulate system was characterized by TEM, HPLC and XPS. Particle size was in the range 426–552 nm and zeta potential values were around +30 mV. This antibacterial coating was able to in vitro inhibit two nosocomial Staphylococcus aureus strains growth, with a reduction of about 20 times compared to controls. No impairment in MG63 osteoblast-like cells viability, adhesion and gene expression were detected at 48 h, 7 and 14 days of culture. Overall, the investigated coating represents a promising candidate for the development of a new antibiotic carrier for titanium implants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|