Chemotherapy induced transient B-cell depletion boosts antibody-forming cells expansion driven by an epidermal growth factor-based cancer vaccine
| Autor: | Rolando Pérez, Enrique Montero, Janet Avellanet, Maikel Valdes, Armando López, Agustin Lage |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_treatment
Lymphocyte Priming (immunology) Oleic Acids Cancer Vaccines Lymphocyte Depletion Mice Immune system Epidermal growth factor medicine Animals Mannitol Doxorubicin Antibody-Producing Cells Cyclophosphamide Cell Proliferation B-Lymphocytes Mice Inbred BALB C Epidermal Growth Factor General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Infectious Diseases medicine.anatomical_structure Immunoglobulin M Immunoglobulin G Immunology biology.protein Molecular Medicine Female Cancer vaccine Antibody Adjuvant Bacterial Outer Membrane Proteins medicine.drug |
| Zdroj: | Vaccine. 27:2230-2239 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2009.02.018 |
| Popis: | Cancer vaccines efficacy may improve inducing a rapid and persistent immune response, early at diagnosis along with standard therapies. EGF chemically conjugated to the carrier protein P64k from Neisseria meningitidis in montanide ISA 51 adjuvant is under evaluation, aiming to stimulate a B-cell response. High-dose cyclophosphamide and doxorubicin after priming enhanced the long-term frequency of EGF-specific antibody-forming cells (AFC) of IgM and IgG isotypes, but not the P64k response. Resulting combination, limitedly operational in Btk deficient xid mice, suggests that preferential B-cell lymphocyte space promoted by cyclophosphamide facilitates remaining EGF-specific AFC undergo homeostatic proliferation driven by boosting, amplifying the response. |
| Databáze: | OpenAIRE |
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