Candidate Gene and MicroRNA Expression in Fetal Membranes and Preterm Delivery Risk
| Autor: | Mahlet G. Tadesse, Daniel A. Enquobahrie, Michelle A. Williams, Mark Hensley, Chunfang Qiu, Dejene F. Abetew, Karin Hevner |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Candidate gene Fetal Membranes Premature Rupture Extraembryonic Membranes Gene Expression Biology Preeclampsia Andrology 03 medical and health sciences 0302 clinical medicine Pre-Eclampsia Pregnancy Risk Factors Placenta microRNA Gene expression medicine Humans Amnion reproductive and urinary physiology Fetus 030219 obstetrics & reproductive medicine Tissue Inhibitor of Metalloproteinase-1 Obstetrics and Gynecology Chorion Original Articles medicine.disease MicroRNAs 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 Immunology embryonic structures Matrix Metalloproteinase 2 Premature Birth Female Premature rupture of membranes Biomarkers |
| Zdroj: | Reproductive sciences (Thousand Oaks, Calif.). 23(6) |
| ISSN: | 1933-7205 |
| Popis: | We investigated candidate gene and microRNA (miRNA) expression in amnion and chorion in relation to risk of preterm delivery (PTD). Amnion and chorion were separated from placenta and collected at delivery from participants who delivered at term (N = 10) and from participants who delivered preterm following spontaneous labor (sPTL-PTD; N = 10), premature rupture of membranes (PPROM-PTD; N = 10), and preeclampsia (PE-PTD; N = 10). Expression of genes (metalloproteinase [MMP] 2, MMP-9, and tissue inhibitors of MMP-1) and miRNAs (miR-199a*, -202*, -210, -214, -223, and -338) was profiled using quantitative real-time polymerase chain reaction approaches. Adjusted multinomial logistic regression models were used to calculate relative risk ratios (RRR), 95% confidence intervals, and P values. Among controls, the expression of miR-199a*, -202*, and -214 was lower in the amnion compared with their expression in the chorion, whereas the expression of miR-210 was higher in the amnion compared with its expression in the chorion (all P values < .05). In the amnion, MMP-9 expression was associated with PTD risk (overall P value = .0092), and MMP-9 expression was positively associated with the risk of PPROM-PTD (RRR: 31.10) and inversely associated with the risk of PE-PTD (RRR:6.55e-6), although individual associations were not statistically significant. In addition, in the amnion, the expression of miR-210 (RRR: 0.45; overall P value = .0039) was inversely associated with the risk of PE-PTD, and miR-223 was inversely associated with all subtypes of PTD (overall P value = .0400). The amnion and chorion differ in their miRNA expression. The expression of MMP-9, miR-210, and -223 in the amnion is associated with PTD risk. |
| Databáze: | OpenAIRE |
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