Acquisition of cell identity in the brown alga Ectocarpus: which of time, cell shape or position matters most?
Autor: | Camille Noûs, Bernard Billoud, Denis Saint-Marcoux, Sabine Chenivesse, Carole Duchêne, Jane A. Langdale, Bénédicte Charrier |
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Přispěvatelé: | Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), University of Oxford [Oxford], Laboratoire Cogitamus, Station biologique de Roscoff, Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0303 health sciences Cell type biology Cellular differentiation Cell [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis Ectocarpus [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] biology.organism_classification 01 natural sciences Phenotype Cell biology Transcriptome 03 medical and health sciences Multicellular organism medicine.anatomical_structure medicine 030304 developmental biology 010606 plant biology & botany Laser capture microdissection |
Popis: | During development, cells undergo simultaneous changes of different types that together depict cell “identity”. In the multicellular brown alga Ectocarpus sp., while ageing, cells change shape and relative position within the filament. Understanding how these factors act and interact to specify cell identity requires markers of cell identity and the ability to genetically separate age, shape and position. Here we used laser capture microdissection (LCM) to isolate specific cell types from young sporophytes of Ectocarpus, and performed differential RNA-seq analysis. Transcriptome profiles of cell types in the wild-type strain provided signatures of the five cell types that can be identified by shape and position. In two mutants, where the relationship between cell shape, position and age are altered, transcriptome signatures revealed that little differential expression could be identified when only shape was perturbed. More generally, although the two mutants are characterised by opposite morphological phenotypes, their transcriptomes were remarkably similar. We concluded that despite the robustness of cell differentiation during WT development, neither the shape nor the position of the cell could serve as a faithful gauge for tracking differentiation. |
Databáze: | OpenAIRE |
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