Sphingosine 1-phosphate signalling through the G-protein-coupled receptor Edg-1
| Autor: | H. Wouter Moolenaar, R. Friso Postma, Ingrid van Etten, C. M. Gerben Zondag, Ingrid Verlaan |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Receptors
Cell Surface Spodoptera Biology Biochemistry Immediate-Early Proteins Receptors G-Protein-Coupled chemistry.chemical_compound Sphingosine Cyclic AMP Enzyme-linked receptor Animals Sphingosine-1-phosphate Receptors Lysophosphatidic Acid Receptor Molecular Biology Cells Cultured G protein-coupled receptor Kinase Nuclear Proteins Zinc Fingers Cell Biology Lipid signaling Cell biology Enzyme Activation Lysophospholipid receptor Receptors Lysophospholipid chemistry Adenylyl Cyclase Inhibitors COS Cells Calcium-Calmodulin-Dependent Protein Kinases Calcium lipids (amino acids peptides and proteins) Lysophospholipids Signal Transduction Transcription Factors Research Article |
| Zdroj: | Biochemical Journal. 330:605-609 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3300605 |
| Popis: | Sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA) are structurally related lipid mediators that act on distinct G-protein-coupled receptors to evoke similar responses, including Ca2+ mobilization, adenylate cyclase inhibition, and mitogen-activated protein (MAP) kinase activation. However, little is still known about the respective receptors. A recently cloned putative LPA receptor (Vzg-1/Edg-2) is similar to an orphan Gi-coupled receptor termed Edg-1. Here we show that expression of Edg-1 in Sf9 and COS-7 cells results in inhibition of adenylate cyclase and activation of MAP kinase (Gi-mediated), but not Ca2+ mobilization, in response to S1P. These responses are specific in that (i) S1P action is not mimicked by LPA, and (ii) Vzg-1/Edg-2 cannot substitute for Edg-1. Thus the Edg-1 receptor is capable of mediating a subset of the cellular responses to S1P. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|