Analysis of the cytokine profile in spleen cells from dogs naturally infected by Leishmania chagasi
| Autor: | Rodolfo Cordeiro Giunchetti, Alexandre Barbosa Reis, L. L. Guerra, Rodrigo Correa-Oliveira, R.S. Lage, Solange C. U. Busek, Guilherme Oliveira |
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| Rok vydání: | 2007 |
| Předmět: |
General Veterinary
Reverse Transcriptase Polymerase Chain Reaction Immunology Parasitism Spleen Leishmaniasis Leishmania chagasi Biology medicine.disease Asymptomatic Dogs Visceral leishmaniasis medicine.anatomical_structure Real-time polymerase chain reaction medicine Splenocyte Animals Cytokines Leishmaniasis Visceral Dog Diseases RNA Messenger Leishmania infantum medicine.symptom |
| Zdroj: | Veterinary Immunology and Immunopathology. 115:135-145 |
| ISSN: | 0165-2427 |
| Popis: | Recent studies suggest that asymptomatic dogs infected with caninevisceral leishmaniasis (CVL) develop a Th1 immunological profile whilst oligosymptomatic and symptomatic CVL-infected animals present a Th2 profile. In the present study, an RT-PCR method has been standardised and employed to evaluate the frequency and the semi-quantitativelevelof expression of the cytokines IL-4, IL-10, IL-12, INF-g and TNF-a in splenocytes of 30 dogs naturally infected with Leishmania chagasi and of 7 non-infected dogs (NID). An increase in the level of expression of IL-12 ( p = 0.059) was detected in all CVL-infected dogs compared with NID. In dogs exhibiting high parasitism, the frequency of expression of IL-10 was higher ( p = 0.011) than in animals presenting low parasitism or medium parasitism (MP) and in NID animals, whilst the level of expression of IL-10 was higher ( p = 0.0094) than in animals exhibiting MP and in the NID group. Positive correlations between the levels of expression of IL-10 with respect to the progression of the disease (IL-10: r = 0.3510; p = 0.0337) and the levels of expression of IL-10 and INF-g increase in parasitism (IL-10: r = 0.3428; p = 0.0438 and INF-g: r = 0.4690; p = 0.0045) were observed. Such data suggest that CVL is marked by a balanced production of Th1 and Th2 cytokines, with a predominant accumulation of IL-10 as a consequence of an increase in parasitic load and progression of the disease, and INF-g was related with the increase in parasitic load. # 2006 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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