Integrated Identification and Quantification of Cyanobacterial Toxins from Pacific Northwest Freshwaters by Liquid Chromatography and High-resolution Mass Spectrometry

Autor:	Theo W. Dreher, Jonathan W. Shepardson, Jeffery T. Morré, Claudia S. Maier, Connie S. Bozarth, Armando Alcazar Magana, Soyoun Ahn
Rok vydání:	2018
Předmět:	chemistry.chemical_classification Detection limit Chromatography biology General Chemistry Microcystin Integrated approach biology.organism_classification Mass spectrometry Algal bloom Article chemistry Microcystis Bloom Quantitative analysis (chemistry)
Zdroj:	Journal of the Mexican Chemical Society. 62
ISSN:	2594-0317 1870-249X
Popis:	The occurrence of harmful algal blooms in nutrient-rich freshwater bodies has increased world-wide, including in the Pacific Northwest. Some cyanobacterial genera have the potential to produce secondary metabolites that are highly toxic to humans, livestock and wildlife. Reliable methods for the detection of cyanobacterial toxins with high specificity and low limits of detection are in high demand. Here we test a relatively new hybrid high resolution accurate mass quadrupole time-of-flight mass spectrometry platform (TripleTOF) for the analysis of cyanobacterial toxins in freshwater samples. We developed a new method that allows the quantitative analysis of four commonly observed microcystin congeners (LR, LA, YR, and RR) and anatoxin-a in a 6-min LC run without solid-phase enrichment. Limits of detection for the microcystin congeners (LR, LA, YR, and RR) and anatoxin-a were Microcystis colonies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c62b32cf9349f172c5350d8d82b81692 https://doi.org/10.29356/jmcs.v62i2.386 Zobrazit plný text záznamu