Prevalence of Bartonella spp. by culture, PCR and serology, in veterinary personnel from Spain
| Autor: | Julie M. Bradley, Xavier Roura, Ricardo G. Maggi, José A. Oteo, Lara García-Álvarez, Aránzazu Portillo, Montserrat San-Martín, Edward B. Breitschwerdt |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Veterinary medicine Bartonella koehlerae Fluorescent Antibody Technique Serology 0302 clinical medicine Seroepidemiologic Studies Bartonella quintana Surveys and Questionnaires Zoonoses Prevalence Dog Diseases Bartonella henselae biology Antibodies Bacterial Bartonella vinsonii berkhoffii Infectious Diseases Female Bartonella Bartonella Infection DNA Bacterial Fastidious organism Genotype 030106 microbiology 030231 tropical medicine Bartonella alphaproteobacteria growth medium Real-Time Polymerase Chain Reaction Veterinary personnel Animal Technicians Veterinarians lcsh:Infectious and parasitic diseases Microbiology 03 medical and health sciences Dogs Bartonella Infections Animals Humans lcsh:RC109-216 Research Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification Virology Cross-Sectional Studies Spain bacteria Parasitology Bacterial antigen |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona RIUR. Repositorio Institucional de la Universidad de La Rioja instname Parasites & Vectors, Vol 10, Iss 1, Pp 1-9 (2017) Parasites & Vectors |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-017-2483-z |
| Popis: | Background The genus Bartonella includes fastidious, facultative intracellular bacteria mainly transmitted by arthropods and distributed among mammalian reservoirs. Bartonella spp. implicated as etiological agents of zoonoses are increasing. Apart from the classical Bartonella henselae, B. bacilliformis or B. quintana, other species (B. elizabethae, B. rochalimae, B. vinsonii arupensis and B. v. berkhoffii, B. tamiae or B. koehlerae, among others) have also been associated with human and/or animal diseases. Laboratory techniques for diagnosis (culture, PCR assays and serology) usually show lack of sensitivity. Since 2005, a method based on a liquid enrichment Bartonella alphaproteobacteria growth medium (BAPGM) followed by PCRs for the amplification of Bartonella spp. has been developed. We aimed to assess culture, molecular and serological prevalence of Bartonella infections in companion animal veterinary personnel from Spain. Methods Each of 89 participants completed a questionnaire. Immunofluorescence assays (IFA) using B. vinsonii berkhoffii (genotypes I, II and III), B. henselae, B. quintana and B. koehlerae as antigens were performed. A cut-off of 1:64 was selected as a seroreactivity titer. Blood samples were inoculated into BAPGM and subcultured onto blood agar plates. Bartonella spp. was detected using conventional and quantitative real-time PCR assays and DNA sequencing. Results Among antigens corresponding to six Bartonella spp. or genotypes, the lowest seroreactivity was found against B. quintana (11.2%) and the highest, against B. v. berkhoffii genotype III (56%). A total of 27% of 89 individuals were not seroreactive to any test antigen. Bartonella spp. IFA seroreactivity was not associated with any clinical sign or symptom. DNA from Bartonella spp., including B. henselae (n = 2), B. v. berkhoffii genotypes I (n = 1) and III (n = 2), and B. quintana (n = 2) was detected in 7/89 veterinary personnel. PCR and DNA sequencing findings were not associated with clinical signs or symptoms. No co-infections were observed. One of the two B. henselae PCR-positive individuals was IFA seronegative to all tested antigens whereas the other one was not B. henselae seroreactive. The remaining PCR-positive individuals were seroreactive to multiple Bartonella spp. antigens. Conclusions High serological and molecular prevalences of exposure to, or infection with, Bartonella spp. were found in companion animal veterinary personnel from Spain. More studies using BAPGM enrichment blood culture and PCR are needed to clarify the finding of Bartonella PCR-positive individuals lacking clinical symptoms. Electronic supplementary material The online version of this article (10.1186/s13071-017-2483-z) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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