Coamplification of simple repetitive DNA fingerprint fragments and the EGFR gene in human gliomas
| Autor: | Hans Zischler, Jörg T. Epplen, Eike Fuhrmann, Dieter Kinzel, Regine Witkowski, Gundula Thiel, Gunter Nisch, Tanka Losanova, Peter Nürnberg |
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| Rok vydání: | 1991 |
| Předmět: |
Adult
Cancer Research Adolescent Biology HaeIII chemistry.chemical_compound Genetics medicine Biomarkers Tumor Humans Repeated sequence Child Gene Southern blot Aged Repetitive Sequences Nucleic Acid Chromosome Aberrations Oligonucleotide Brain Neoplasms Carcinoma Gene Amplification DNA Neoplasm Glioma Middle Aged Molecular biology DNA Fingerprinting Clone Cells ErbB Receptors chemistry DNA profiling Oligomer restriction DNA Polymorphism Restriction Fragment Length medicine.drug Medulloblastoma |
| Zdroj: | Genes, chromosomescancer. 3(2) |
| ISSN: | 1045-2257 |
| Popis: | DNA fingerprints were generated by the oligonucleotide probe (GTG)5 from surgically removed tissue and/or primary cell culture of 36 intracranial tumors (31 gliomas, 1 medulloblastoma, 4 metastatic carcinomas) and compared with the constitutional banding pattern obtained from the peripheral blood leukocytes of each patient. A multitude of somatic changes was detected and found to reflect the chromosome alterations identified by parallel karyotype analysis. Gain and/or loss of bands or significant band intensity shifts could be demonstrated in the fingerprints of more than 80% of the tumors investigated. This included a highly amplified fingerprint fragment in five independent gliomas (four of them had double minutes, dmin) which appeared not individual- but tumor-specific (2.4 kilobases, kb, after HaeIII digestion). Rehybridization with the oligonucleotide probes (GT)8 and (GATA)4, respectively, revealed additional amplified fingerprint fragments in the tumor DNA of these patients. While a (ca/gt)n fragment (2.6 kb. HaeIII) was also found to be amplified in all five cases, one band detected with (GATA)4 (1.4 kb, HaeIII) represented a unique feature for one of these tumors only. Amplification of the epidermal growth factor receptor (EGFR) gene via Southern blot hybridization was revealed only in those tumors showing the amplified DNA fingerprint fragments as well. Thus in many gliomas the amplification unit harbors two simple repetitive DNA fingerprint loci, (cac/gtg)n and (ca/gt)n, in addition to the EGFR gene. |
| Databáze: | OpenAIRE |
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