| Popis: |
The present state-of-the-art of the Uppsala “NMR-window” concept (see refs 1-4, 7-8 & 10-11) developed for the high-field NMR conformational studies on the solution structure of large, biologically functional DNAs and RNAs is summarised. The use of specifically deuterated nucleotide building blocks allows the extraction of the vital J coupling and nOe information to give the NMR-constrained structure refinements. In particular, the improved preparation of C5-deuterio uridine and cytidine blocks (i.e. hexadeuterated block) is described for their use in the “NMR-window” concept. |
