Bacterial communities in the gut of wild and mass-reared Zeugodacus cucurbitae and Bactrocera dorsalis revealed by metagenomic sequencing
| Autor: | Ramesh S. Hire, Suresh K. G. Shettigar, Ashok B. Hadapad |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Microbiology (medical) DNA Bacterial Male Firmicutes lcsh:QR1-502 Zoology Bactrocera sp 01 natural sciences Microbiology Bactrocera dorsalis DNA Ribosomal Mass rearing lcsh:Microbiology 03 medical and health sciences Sterile insect technique Tephritidae RNA Ribosomal 16S High throughput technology Bactrocera Animals Gut bacteria Phylogeny 030304 developmental biology Melon fly 0303 health sciences Mangifera biology Bacteria Sequence Analysis RNA Research fungi Bacteroidetes High-Throughput Nucleotide Sequencing food and beverages biology.organism_classification Gastrointestinal Microbiome 010602 entomology Cucurbitaceae SIT Female Bacterial endosymbionts Metagenomic sequencing |
| Zdroj: | BMC Microbiology, Vol 19, Iss S1, Pp 1-11 (2019) BMC Microbiology |
| ISSN: | 1471-2180 |
| Popis: | BackgroundInsect pests belonging to genus Bactrocera sp. (Diptera: Tephritidae) pose major biotic stress on various fruits and vegetable crops around the world. Zeugodacus and Bactrocera sp. are associated with diverse bacterial communities which play an important role in the fitness of sterile insects. The wild populations of melon fly, Zeugodacus cucurbitae (Coquillett) and Oriental fruit fly,Bactrocera dorsalis(Hendel) were collected from pumpkin and mango fields, respectively. The laboratory populations of Z. cucurbitae andB. dorsaliswere mass-reared on bottle gourd and sweet banana, respectively. Bacterial communities present in the gut of wild and mass-reared mature (~ 12 days old) and newly emerged (Z. cucurbitaeandB. dorsaliswere assessed. We used Illumina HiSeq next-generation sequencing of16S rRNAgene to profile the gut bacterial communities of wild and mass-reared mature and newly emergedZ. cucurbitaeandB. dorsalisadults.ResultsWe found diverse bacterial composition in the gut of wild and mass-rearedZ. cucurbitae(ZC) andB. dorsalis(BD) with varied relative abundance. Few taxonomic groups were common to both the species. The most dominant phyla in all samples ofZ. cucurbitaeandB. dorsalisadults were Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. The phylum Proteobacteria occurred more in wildZ. cucurbitae(~ 87.72%) andB. dorsalis(~ 83.87%) as compared to mass-rearedZ. cucurbitae(64.15%) andB. dorsalis(~ 80.96%). Higher relative abundance of Phylum Firmicutes was observed in mass-reared fruit fly than wild adults. Cyanobacteria/Chloroplast and Actinobacteria were also present with very low relative abundance in both wild as well as mass-reared melon fly and Oriental fruit fly. Enterobacteriaceae (61.21%) was dominant family in the gut of both wild and mass-reared adults.ProvidenciaandLactococcuswere dominant genera with varied relative abundance in wild as well as in mass-reared mature and newly emerged fruit fly adults of both species. Some of the genera likeMorganellaandSerratiawere only detected in mass-reared mature and newly emergedZ. cucurbitaeandB. dorsalisadults. Principal Coordinate Analysis (PCoA) showed that fruit fly adult samples were grouped based on species and age of the adults while no grouping was observed on the basis of sex of the adult fruit fly.ConclusionsThe gut bacterial communities associated with wild and mass-reared mature and newly emerged adults ofZ. cucurbitaeandB. dorsalisshowed variation that depends on species and age of the insects. Understanding the gut microbiota of wild and mass-rearedZ. cucurbitaeandB. dorsalisusing high throughput technology will help to illustrate microbial diversity and this information could be used to develop efficient mass-rearing protocols for successful implementation of sterile insect technique (SIT). |
| Databáze: | OpenAIRE |
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