The transduction of mechanical force into biochemical events in bone cells may involve activation of phospholipase A2
| Autor: | Itzhak Binderman, Zvi Shimshoni, Arie Harell, Uriel Zor, Alvin M. Kaye, Dalia Somjen |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Stimulation Arachidonic Acids Cyclase Dinoprostone Phospholipases A Membrane Lipids chemistry.chemical_compound Endocrinology Phospholipase A2 Internal medicine Bone cell Cyclic AMP medicine Animals Orthopedics and Sports Medicine Prostaglandin E2 Cells Cultured Phospholipase C biology Prostaglandins E Skull Rats Cell biology Enzyme Activation Phospholipases A2 chemistry Phospholipases Cell culture biology.protein Arachidonic acid Stress Mechanical medicine.drug |
| Zdroj: | Calcified Tissue International. 42:261-266 |
| ISSN: | 1432-0827 0171-967X |
| DOI: | 10.1007/bf02553753 |
| Popis: | Mechanical forces applied to cultured bone cells induce the production of cAMP via stimulation of the formation of prostaglandin E2 (PGE2) and its release into the medium, resulting in stimulation of adenylate cyclase. In this paper we show that either the antibiotic gentamycin (100 micrograms/ml) or antiphospholipid antibodies (0.1%) which bind to membrane phospholipids abolish cAMP formation induced by mechanical forces; exogenously added arachidonic acid or PGE2 stimulates cAMP formation, even in the presence of these agents. Addition of exogenous phospholipase A2 (but not phospholipase C) causes an increase in the formation of cAMP in bone cells, a response that is also inhibited by gentamycin or antiphospholipase antibodies. These observations suggest that mechanical forces exert their effect on bone cells via the following chain of events: (1) activation of phospholipase A2, (2) release of arachidonic acid, (3) increased PGE synthesis, (4) augmented cAMP production. |
| Databáze: | OpenAIRE |
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