KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis
| Autor: | Sabine Müller, Kenneth W. Berendzen, Pantelis Livanos, Leander Rohr, Elisabeth Lipka, Steffi Zimmermann, Arvid Herrmann |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Kinesins Plant Science macromolecular substances Biology Breakthrough Report Phragmoplast 01 natural sciences Microtubules Prophase Motor protein 03 medical and health sciences Kinetochores Metaphase Kinetochore Spindle midzone Cell Biology Cell biology Spindle apparatus 030104 developmental biology Multipolar spindles Cytokinesis 010606 plant biology & botany |
| Zdroj: | Plant Cell |
| ISSN: | 1532-298X |
| Popis: | The bipolar mitotic spindle is a highly conserved structure among eukaryotes that mediates chromosome alignment and segregation. Spindle assembly and size control are facilitated by force-generating microtubule-dependent motor proteins known as kinesins. In animals, kinesin-12 cooperates with kinesin-5 to produce outward-directed forces necessary for spindle assembly. In plants, the relevant molecular mechanisms for spindle formation are poorly defined. While an Arabidopsis thaliana kinesin-5 ortholog has been identified, the kinesin-12 ortholog in plants remains elusive. In this study, we provide experimental evidence for the function of Arabidopsis KINESIN-12E in spindle assembly. In kinesin-12e mutants, a delay in spindle assembly is accompanied by the reduction of spindle size, demonstrating that KINESIN-12E contributes to mitotic spindle architecture. Kinesin-12E localization is mitosis-stage specific, beginning with its perinuclear accumulation during prophase. Upon nuclear envelope breakdown, KINESIN-12E decorates subpopulations of microtubules in the spindle and becomes progressively enriched in the spindle midzone. Furthermore, during cytokinesis, KINESIN-12E shares its localization at the phragmoplast midzone with several functionally diversified Arabidopsis KINESIN-12 members. Changes in the kinetochore and in prophase and metaphase spindle dynamics occur in the absence of KINESIN-12E, suggest it might play an evolutionarily conserved role during spindle formation similar to its spindle-localized animal kinesin-12 orthologs. |
| Databáze: | OpenAIRE |
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