Phenotypical and functional differences in germinative subpopulations derived from normal and psoriatic epidermis
| Autor: | Peter C.M. van de Kerkhof, M.E.J. Franssen, Joost Schalkwijk, Gerty Vierwinden, Patrick L.J.M. Zeeuwen, Piet E.J. van Erp |
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| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty proliferation Biopsy Cellular differentiation Population Immunocytochemistry Dermatology Biology Polymerase Chain Reaction Auto-immunity transplantation and immunotherapy [N4i 4] Biochemistry Flow cytometry stem cells Translational research [ONCOL 3] medicine Humans Psoriasis RNA Messenger education Molecular Biology Cells Cultured Chronic inflammation and autoimmunity [UMCN 4.2] cell culture education.field_of_study medicine.diagnostic_test Epidermis (botany) Integrin beta1 Cell Biology Flow Cytometry Cell biology transit amplifying cells Pathogenesis and modulation of inflammation [N4i 1] Ki-67 Antigen Phenotype Cell culture Epidermis Stem cell Infection and autoimmunity [NCMLS 1] Biomarkers Cell Division Elafin |
| Zdroj: | Journal of Investigative Dermatology, 124, 373-83 Journal of Investigative Dermatology, 124, 2, pp. 373-83 |
| ISSN: | 0022-202X |
| Popis: | Contains fulltext : 49144.pdf (Publisher’s version ) (Closed access) A model that explains how maintenance of normal homeostasis in human epidermis is achieved describes a heterogeneous cell population of stem cells (SC) and transit amplifying cells (TAC). There must be a tightly regulated balance between SC self-renewal and the generation of TAC that undergo a limited number of divisions before giving rise to postmitotic, terminally differentiated cells. To investigate whether this balance is disturbed in psoriatic epidermis, we have characterized flow sorted enriched SC and TAC using immunocytochemistry, flow cytometry, and real-time quantitative PCR. Our data demonstrate phenotypical and functional differences in SC (beta(1)-integrin bright) and TAC (beta(1)-integrin dim) enriched fractions between normal and psoriatic keratinocytes. Some of these were expected, such as mRNA levels of keratins 6 and 10 and of the Ki-67 antigen. Most remarkable were differences in phenotype of the psoriatic TAC compared with TAC from normal skin. These subpopulations also displayed striking differences following culture. Downregulation of markers associated with the regenerative phenotype (psoriasin, elafin, psoriasis-associated fatty acid binding protein) in cultured psoriatic dim cells in the absence of hyperproliferation suggests that proliferation and regenerative maturation are coupled. From these results, in combination with our earlier findings, we propose a model for epidermal growth control in which TAC play a crucial role. |
| Databáze: | OpenAIRE |
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