Design, Fabrication, and In Vitro Evaluation of Nanoceria-Loaded Nanostructured Lipid Carriers for the Treatment of Neurological Diseases
| Autor: | Nadia Garino, Matteo Battaglini, Gianni Ciofani, Andrea Ancona, Francisco Palazon, Doriana Debellis, Attilio Marino, Valentina Alice Cauda, Ivana Cavaliere, Christos Tapeinos |
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| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
medicine.medical_treatment Sonication nanostructured lipid carriers 0206 medical engineering Biomedical Engineering 02 engineering and technology Blood–brain barrier Neuroprotection lipids Biomaterials antioxidants blood-brain barrier cerium oxide nanoparticles cerium oxide nanoparticles antioxidants blood-brain barrier nanostructured lipid carriers medicine Chemistry Neurodegeneration differentiation 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering In vitro medicine.anatomical_structure Biophysics encapsulation nanoparticles Ceo2 nanoparticles 0210 nano-technology Large group |
| Zdroj: | Battaglini, M, Tapeinos, C, Cavaliere, I, Marino, A, Ancona, A, Garino, N, Cauda, V, Palazon, F, Debellis, D & Ciofani, G 2019, ' Design, Fabrication, and in Vitro Evaluation of Nanoceria-Loaded Nanostructured Lipid Carriers for the Treatment of Neurological Diseases ', ACS Biomaterials Science and Engineering, vol. 5, no. 2, pp. 670–682 . https://doi.org/10.1021/acsbiomaterials.8b01033 ACS Biomaterials Science & Engineering |
| Popis: | Neurodegenerative diseases comprise a large group of disorders characterized by a dramatic synaptic connections loss, occurring as a result of neurodegeneration, which is closely related to the overproduction of reactive oxygen and nitrogen species. Currently, the treatment of neurodegenerative diseases has been limited mainly because of the inability of the synthesized delivery systems to cross the blood-brain barrier and to successfully deliver their therapeutic cargo to the diseased tissue. Taking into consideration the aforementioned limitations, we designed a lipid-based nanotherapeutic vector composed of biomimetic lipids and CeO2nanoparticles (nanoceria, NC). NC have shown to be a promising tool for the treatment of several pathological conditions ranging from cancer to neurological diseases, mainly because of their antioxidant properties, while lipid-based structures have been shown to have an inherent ability to cross the blood-brain barrier. The lipid-based nanotherapeutics were successfully fabricated using a combination of ultrasonication and high-pressure homogenization techniques, and they were fully characterized morphologically and physicochemically. Their antioxidant ability was demonstrated using electron paramagnetic resonance spectroscopy and antioxidant assays. These innovative nanotherapeutics demonstrated a higher colloidal stability with respect to free NC, preserving at the same time their antioxidant properties. Finally, the ability of the lipid carriers to cross a model of the blood-brain barrier and to be internalized by neurons, acting both as neuroprotective and pro-neurogenic agents, was demonstrated using single- and triple-culture systems. |
| Databáze: | OpenAIRE |
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