Hypericin inclusion complexes encapsulated in liposomes for antimicrobial photodynamic therapy
| Autor: | Udo Bakowsky, Benjamin Sebastian Seitz, Nikola Plenagl, Jana Brüßler, Jarmila Jedelská, Lili Duse, Shashank Reddy Pinnapireddy |
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| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
Drug Compounding Pharmaceutical Science Photodynamic therapy 02 engineering and technology 030226 pharmacology & pharmacy Polyethylene Glycols 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Perylene Anthracenes Liposome Photosensitizing Agents Vesicle Phosphatidylethanolamines beta-Cyclodextrins technology industry and agriculture Biofilm 021001 nanoscience & nanotechnology Antimicrobial Hypericin Bioavailability Anti-Bacterial Agents chemistry Photochemotherapy Biofilms Staphylococcus saprophyticus Liposomes Biophysics 0210 nano-technology Phototoxicity |
| Zdroj: | International journal of pharmaceutics. 570 |
| ISSN: | 1873-3476 |
| Popis: | The naturally occurring anthraquinone derivative hypericin is a highly potent photosensitiser. Several in vitro studies show high phototoxicity of the pigment towards gram-positive bacteria. Nevertheless, the highly lipophilic nature and poor bioavailability prevent its application in daily clinical practice thus leading to a limited therapeutic value of hypericin. Liposomal encapsulation could help overcome these limitations and would make hypericin available for daily clinical practice. The use of liposomes as carriers for hypericin in antimicrobial photodynamic therapy (aPDT) is quite new. The aim of this work was to improve the photodynamic efficiency of the previously mentioned carriers by entrapping hypericin in the aqueous compartment of the liposomes. Therefore, a water-soluble inclusion complex of hypericin and (2-hydroxypropyl)-beta-cyclodextrin (Hyp-HPβCD) was prepared. After encapsulation of the inclusion complex into DSPC and DSPC/DPPC/DSPE-PEG liposomes with the dehydration-rehydration vesicle (DRV) method, the formulations were physicochemical characterised. The photodynamic efficiency towards the gram-positive model strain Staphylococcus saprophyticus subsp. bovis. was tested on planktonic cells as well as on biofilms. DSPC liposomes achieved a 4.1log reduction and the DSPC/DPPC/DSPE-PEG liposomes a 2.6log reduction in growth of planktonic bacteria, while Hyp-HPβCD showed total eradication. Even bacterial cells growing in a biofilm could be treated effectively in vitro. |
| Databáze: | OpenAIRE |
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