Thermodynamic analysis of protein interactions with biosensor technology
| Autor: | Robert Karlsson, Anette Persson, Håkan Roos, Helena Nilshans |
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| Rok vydání: | 1998 |
| Předmět: |
Isothermal microcalorimetry
Kinetics Enthalpy HIV Core Protein p24 Thermodynamics Biosensing Techniques Calorimetry HIV Antibodies In Vitro Techniques Fatty Acid-Binding Proteins Kinetic energy Antigen-Antibody Reactions Entropy (classical thermodynamics) Transition state theory Reaction rate constant Structural Biology Animals Humans Molecular Biology Chemistry Antibodies Monoclonal Proteins Muramidase Carrier Proteins Biosensor Protein Binding |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1099-1352 0952-3499 |
| DOI: | 10.1002/(sici)1099-1352(199812)11:1/6<204::aid-jmr424>3.0.co;2-t |
| Popis: | A methodology using biosensor technology for combined kinetic and thermodynamic analysis of biomolecular interactions is described. Rate and affinity constants are determined with BIAcore. Thermodynamics parameters, changes in free energy, enthalpy and entropy, are evaluated from equilibrium data and by using rate constants and transition state theory. The methodology using van't Hoff theory gives complementary information to microcalorimetry, since only the direct binding is measured with BIAcore whereas microcalorimetry measures all components, including e.g. hydration effects. Furthermore, BIAcore gives possibilities to gain new information by thermodynamic analysis of the rate constants. |
| Databáze: | OpenAIRE |
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