Tyr-TGFα transgenic mice develop ocular melanocytic lesions
| Autor: | Rebecca S. Mason, G. P. M. Moore, Clare Gordon-Thomson, I. A. Cree, R. Sutton |
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| Rok vydání: | 2002 |
| Předmět: |
Genetically modified mouse
Cancer Research Pathology medicine.medical_specialty TGF alpha Transgene Mice Transgenic Dermatology Melanocyte Biology Eye Mice medicine Animals Tissue Distribution Transgenes Promoter Regions Genetic Melanoma Retinal pigment epithelium Models Genetic integumentary system Monophenol Monooxygenase Eye Neoplasms Transforming Growth Factor alpha medicine.disease Epithelium Cell Transformation Neoplastic medicine.anatomical_structure Oncology Melanocytes sense organs Choroid |
| Zdroj: | Melanoma Research. 12:435-439 |
| ISSN: | 0960-8931 |
| DOI: | 10.1097/00008390-200209000-00004 |
| Popis: | Transforming growth factor-alpha (TGFalpha) has been implicated in melanocyte transformation, as it is expressed in melanocytic lesions and in melanoma cells. We investigated its role in melanoma development using a transgenic mouse model. The mice were generated by microinjection of a transgene with 270 bp of the mouse tyrosinase promoter and the cDNA for human TGFalpha. No significant skin abnormalities were found, but individuals from three transgenic lines developed ocular melanocytoses (seven out of 10 transgenics), usually after a long latency period. In particular, the melanocyte component of the choroid was thicker than in non-transgenic controls, consistent with hyperplasia. The retinal pigment epithelium was unaffected. Melanocytic lesions were also present in the posterior eye, and abnormal distributions of melanocytes were found in neural tissue of the brain, skeletal muscle of the head and the Harderian glands, indicating migration from the choroid. It was concluded that mice engineered to express the normal growth factor TGFalpha from a tyrosinase promoter spontaneously developed melanocytic lesions in the eye but not the skin. |
| Databáze: | OpenAIRE |
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