High osmotic stress improves electro-transformation efficiency of fission yeast
| Autor: | Minoru Suga, Isamu Kusanagi, Toyomasa Hatakeyama |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Osmotic concentration
Osmotic shock Sodium Osmolar Concentration Colony Count Microbial chemistry.chemical_element Sodium Chloride Biology Osmosis Microbiology Yeast chemistry.chemical_compound Electroporation Transformation Genetic Biochemistry chemistry Osmotic Pressure Schizosaccharomyces Genetics Biophysics Sorbitol Osmotic pressure Molecular Biology Transformation efficiency |
| Zdroj: | FEMS Microbiology Letters. 225:235-239 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1016/s0378-1097(03)00518-4 |
| Popis: | A preincubation of fission yeast cells with hyperosmotic solution improved the electro-transformation efficiency. The efficiency increased approximately five-fold when the cells were preincubated with 2.0 M sorbitol and 1.5 M NaCl at 30 degrees C for 60 min before an applied high electric pulse. Losses in the efficiency of the cells after hyperosmotic stress above 2.5 M sorbitol and 2.0 M NaCl were directly related to the marked reduction of viability. The efficiency at 2.0 M sorbitol gradually increased until 60 min of the preincubation period, but longer exposure resulted in a gradual decrease. On the other hand, when the cells of the osmotic-sensitive mutant were preincubated with isosmotic solution of 0.5 M sorbitol, the efficiency was also dramatically increased by approximately 15-fold. These improvements in efficiency were observed in sublethal conditions of osmotic stress regardless of osmoticums and strains. |
| Databáze: | OpenAIRE |
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