Complement proteins regulating macrophage polarisation on biomaterials

Autor: Mikel Azkargorta, Yang Zhang, Mariló Gurruchaga, J. J. J. P. van den Beucken, J. Suay, J. J. Martín de Llano, Cristina Martínez-Ramos, Isabel Goñi, Nuno Araújo-Gomes, Francisco Romero-Gavilán, Felix Elortza
Rok vydání: 2019
Předmět:
Proteomics
Cell
Biocompatible Materials
02 engineering and technology
01 natural sciences
immune response
Mice
Colloid and Surface Chemistry
CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA
Titanium
010304 chemical physics
Chemistry
hybrid sol-gel
Biomaterial
Surfaces and Interfaces
General Medicine
Silanes
021001 nanoscience & nanotechnology
Interleukin-10
medicine.anatomical_structure
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
Rabbits
0210 nano-technology
Biotechnology
Complement system
Biocompatibility
Surface Properties
Macrophage polarization
macrophage plasticity
Osseointegration
Hybrid sol-gel
Macrophage plasticity
Immune system
All institutes and research themes of the Radboud University Medical Center
proteomics
dental implants
0103 physical sciences
medicine
Animals
Secretion
Particle Size
Physical and Theoretical Chemistry
Immune response
complement system
Tibia
Tumor Necrosis Factor-alpha
Macrophages
Dental implants
Complement System Proteins
RAW 264.7 Cells
Biophysics
Zdroj: Repositori Universitat Jaume I
Universitat Jaume I
Colloids and Surfaces B-Biointerfaces, 181, pp. 125-133
Colloids and Surfaces B-Biointerfaces, 181, 125-133
COLLOIDS AND SURFACES B-BIOINTERFACES
r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
ISSN: 0927-7765
Popis: [EN] One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-alpha and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.
This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Predoc/2014/25, UJI-B2017-37]; Basque Government [IT611-13, Predoc/2016/1/0141]; University of the Basque Country [UFI11/56]; CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), ProteoRed-ISCIII [PRB3 IPT17/0019]; CIBERehd Network and Severo Ochoa Grant [SEV-2016-0644]. Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), René van Rheden, Vicent Cuijpers (Radboudumc) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.
Databáze: OpenAIRE
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