Degradation of subchondral bone collagen in the weight-bearing area of femoral head is associated with osteoarthritis and osteonecrosis
| Autor: | De-Yi Yan, Jiahao Tang, Zong-Yi Wu, Zijian Shen, She-Ji Weng, Bing-Li Bai, Bingzhang Wang, Lei Yang, Liang Chen, Jingdong Zhang, Zhongjie Xie |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Pathology lcsh:Diseases of the musculoskeletal system Collagen fibers H&E stain 030209 endocrinology & metabolism Osteoarthritis Weight-Bearing Masson's trichrome stain 03 medical and health sciences Femoral head 0302 clinical medicine lcsh:Orthopedic surgery Femur Head Necrosis medicine Humans Orthopedics and Sports Medicine Aged Femoral neck business.industry Cartilage Osteonecrosis Femur Head Subchondral bone Middle Aged medicine.disease Staining lcsh:RD701-811 030104 developmental biology medicine.anatomical_structure Cancellous Bone Proteolysis Orthopedic surgery Female Surgery Collagen lcsh:RC925-935 business Research Article |
| Zdroj: | Journal of Orthopaedic Surgery and Research Journal of Orthopaedic Surgery and Research, Vol 15, Iss 1, Pp 1-9 (2020) |
| ISSN: | 1749-799X |
| DOI: | 10.1186/s13018-020-02065-y |
| Popis: | Background The aim of the study was to evaluate the change of subchondral bone collagen and trabecular bone in the weight-bearing area of femoral head from patients with osteoarthritis (OA) or osteonecrosis of femoral head (ONFH), and discuss the effect of collagen degradation on OA and ONFH. Methods Femoral heads from patients with femoral neck fracture (FNF) were collected as control group. All collected samples were divided into OA group (N = 10), ONFH group (N = 10), and FNF group (N = 10). Differences of subchondral bone collagen were compared through scanning electron microscope (SEM) observation, immunohistochemistry staining, and Masson’s trichrome staining. Alteration of subchondral bone was displayed through hematoxylin and eosin (H&E) staining and gross morphology. Results SEM results showed that collagen fibers in OA and ONFH group appeared to be thinner, rougher, sparser, and more wizened. Immunohistochemistry and Masson’s trichrome staining results demonstrated that the content of collagen fibers in the OA and ONFH group was obviously less than the FNF group. H&E staining results showed that trabecular bone in OA and ONFH group appeared to be thinner and ruptured. Gross morphology results showed that the degeneration and destruction of cartilage and subchondral bone in OA and ONFH group were severer than FNF group. The characteristics mentioned above in ONFH group were more apparent than OA group. Conclusions This study revealed that degradation of collagen fibers from subchondral bone in the weight-bearing area of femoral head was associated with OA and ONFH, which may help to find new therapeutic strategies of the diseases. |
| Databáze: | OpenAIRE |
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