Three-dimensional structure of outer hair cell pillars
| Autor: | Miriam von Tiedemann, Britta Flock, Lars-Göran Öfverstedt, Ulf Skoglund, Anders Fridberger, Åke Flock |
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| Rok vydání: | 2009 |
| Předmět: |
Electron Microscope Tomography
Materials science Guinea Pigs General Medicine Anatomy law.invention Cell membrane Hair Cells Auditory Outer Imaging Three-Dimensional Membrane medicine.anatomical_structure Otorhinolaryngology Electron tomography law Thin segment medicine Animals sense organs Hair cell Electron microscope Cytoskeleton Cochlea Biomedical engineering |
| Zdroj: | Acta Oto-Laryngologica. 129:940-945 |
| ISSN: | 1651-2251 0001-6489 |
| DOI: | 10.1080/00016480802552519 |
| Popis: | Conclusions. Electron tomography was used to generate three-dimensional reconstructions of the pillars that connect the cell membrane with the cytoskeleton of the outer hair cell. Results are consistent with the hypothesis that pillars are important for mechanically linking the membrane with the cytoskeleton. Objective: To make a qualitative assessment of the morphology of the sub-membrane pillars of cochlear outer hair cells. Materials and methods. Guinea pig cochleae were fixed and prepared for electron microscopy using protocols described previously. Sections were imaged on an electron microscope equipped with a goniometer. The specimens were tilted through a range of 120°, and an image was acquired at each tilt angle. Filtered back-projection was used to generate three-dimensional reconstructions. Results. Twelve individual pillars were successfully reconstructed. Pillars often connect to the cell membrane through a thin segment, and to the cytoskeleton through a forking structure that may form a cent... |
| Databáze: | OpenAIRE |
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