Taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as Pseudomonas putida
| Autor: | Christelle Gruffaz, Daniel Izard, Topi Tulkki, Jean-Marie Meyer |
|---|---|
| Přispěvatelé: | Génétique moléculaire, génomique, microbiologie (GMGM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2007 |
| Předmět: |
Pseudomonas monteilii
Pseudomonas mandelii Iron Biovar Molecular Sequence Data Pseudomonas jessenii Siderophores [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Microbiology Pseudomonas mosselii Pseudomonas Environmental Microbiology Amino Acid Sequence Ecology Evolution Behavior and Systematics Pseudomonas putida Nucleic Acid Hybridization Biological Transport General Medicine biology.organism_classification Bacterial Typing Techniques bacteria Isoelectric Focusing Oligopeptides Pseudomonadaceae |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2007, 57 (Pt 11), pp.2543-56. ⟨10.1099/ijs.0.65233-0⟩ |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.65233-0 |
| Popis: | One hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as Pseudomonas putida (83 strains), P. putida biovar A (49 strains), P. putida biovar B (10 strains) and P. putida biovar C (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. Both methods demonstrated a great diversity among these strains, which could be subdivided into 35 siderovars. Some siderovars specifically included strains that have subsequently been transferred to well-defined Pseudomonas species, e.g. Pseudomonas monteilii or Pseudomonas mosselii, or which could be related by their siderotype to Pseudomonas jessenii or Pseudomonas mandelii. Other siderovars included strains sharing a high level of DNA-DNA relatedness (>70 %), thus demonstrating that siderotyping could easily circumscribe strains at the species level. However, a group of seven strains, including the type strain, P. putida ATCC 12633T, were allocated into four siderovars, despite sharing DNA–DNA relatedness values of higher than 70 %. Interestingly, the strong genomic relationships between these seven strains were supported by the structural relationships among their pyoverdines, thus reflecting their phylogenetic affinities. These results strongly support the view that pyoverdine-based siderotyping could be used as a powerful tool in Pseudomonas taxonomy. |
| Databáze: | OpenAIRE |
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