Pro-fibrotic effect of oxidized LDL in cardiac myofibroblasts
| Autor: | Lorena García, Paulina Cerda-Opazo, Andrew F. G. Quest, Valeria Garrido-Moreno, Jorge R. Toledo, Danica Jimenez-Gallegos, Mario Chiong, Monica Villa |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell type Cardiac fibrosis Cell Biophysics Biochemistry Collagen Type I Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Fibrosis Cell Movement medicine Animals Receptor Myofibroblasts Molecular Biology Cell Proliferation biology Chemistry Myocardium Cell Biology medicine.disease Scavenger Receptors Class E Cell biology Extracellular Matrix Fibronectin Lipoproteins LDL 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis cardiovascular system biology.protein lipids (amino acids peptides and proteins) Myofibroblast Lipoprotein |
| Zdroj: | Biochemical and biophysical research communications. 524(3) |
| ISSN: | 1090-2104 |
| Popis: | Inflammatory signals associated with cardiac diseases trigger trans-differentiation of cardiac fibroblasts to cardiac myofibroblasts. Cardiac myofibroblasts are the main cell type involved in the development of cardiac fibrosis, a diffuse and disproportionate accumulation of collagen in the myocardium. Although the role of the scavenger like-lectin receptor LOX-1 was previously investigated in cardiac fibroblasts and fibrosis, the involvement of the LOX-1 ligand -oxidized low-density lipoprotein (oxLDL)- on cardiac myofibroblast function still remains unexplored. In the present work, we investigated the effect of oxLDL/LOX-1 on fibrotic markers and cardiac myofibroblast function. Our in vitro results showed that oxLDL increased cardiac myofibroblast proliferation, triggered an increase in the synthesis of collagen type I and fibronectin containing extra domain A, and stimulated collagen type I secretion. oxLDL also decreased cardiac myofibroblast migration, collagen gel contraction and cell area, without modifying α-smooth muscle actin protein levels. These effects were dependent on LOX-1, because LOX-1 knockdown abolished oxLDL effects. Collectively these data showed that oxLDL has important modulatory effects on cardiac myofibroblast function. |
| Databáze: | OpenAIRE |
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