Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato
| Autor: | Yuanyuan Lin, Junchao Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
WT wild type Chl Chlorococcum sp Algae LCYB Lycopene β-cyclase Mannose Plant Science Biology 010603 evolutionary biology 01 natural sciences Tomato Transformation MS Murashige and Skoog RACE Rapid amplification of cDNA ends UPLC Ultra-performance liquid chromatography chemistry.chemical_compound Chlorococcum lcsh:Botany Gene cluster Gene lcsh:QH301-705.5 Ecology Evolution Behavior and Systematics Selectable marker BHY β-carotene hydroxylase fungi Astaxanthin food and beverages PMI phosphomannose isomerase PSY phytoene synthase biology.organism_classification BKT β-carotene ketolase Genetically modified organism lcsh:QK1-989 Transformation (genetics) Biochemistry chemistry lcsh:Biology (General) Callus Phosphomannose isomerase PCR Polymerase chain reaction 010606 plant biology & botany Research Paper |
| Zdroj: | Plant Diversity, Vol 43, Iss 1, Pp 63-70 (2021) Plant Diversity |
| ISSN: | 2468-2659 |
| Popis: | Establishing a transgenic plant largely relies on a selectable marker gene that can confer antibiotic or herbicide resistance to plant cells. The existence of such selectable marker genes in genetically modified foods has long been criticized. Plant cells generally exhibit too low an activity of phosphomannose isomerase (PMI) to grow with mannose as a sole carbon source. In this study, we characterized PMI from the green microalga Chlorococcum sp. and assessed its feasibility as a selectable marker for plant biotechnology. Chlorococcum sp. PMI (ChlPMI) was shown to be closely related to higher plants but more distant to bacterial counterparts. Overexpression of ChlPMI in tomato induced callus and shoot formation in media containing mannose (6 g/L) and had an average transformation rate of 3.9%. Based on this transformation system, a polycistronic gene cluster containing crtB, HpBHY, CrBKT and SlLCYB (BBBB) was co-expressed in a different tomato cultivar. Six putative transformants were achieved with a transformation rate of 1.4%, which produced significant amounts of astaxanthin due to the expression of the BBBB genes. Taken together, these findings indicate that we have established an additional tool for plant biotechnology that may be suitable for genetically modifying foods safely. |
| Databáze: | OpenAIRE |
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