dRTEL1 is essential for the maintenance of Drosophila male germline stem cells

Autor: Ruiyan Kong, Feng Guang Goh, Gary R. Hime, Ying Yang, W. Gregory Somers, Zhouhua Li, Yu Cai
Rok vydání: 2021
Předmět:
Male
Life Cycles
Cancer Research
DNA Repair
Cellular differentiation
Regulator
Gene Expression
QH426-470
Biochemistry
Germline
Transcriptome
Larvae
Animal Cells
Medicine and Health Sciences
Drosophila Proteins
Testes
Cell Self Renewal
Genetics (clinical)
Stem Cells
Drosophila Melanogaster
Eukaryota
Cell Differentiation
Animal Models
Cell biology
Insects
Nucleic acids
Ovaries
Experimental Organism Systems
Drosophila
Female
Anatomy
Cellular Types
Stem cell
Genital Anatomy
Signal Transduction
Research Article
endocrine system
Arthropoda
DNA repair
Biology
Research and Analysis Methods
Model Organisms
Genetics
Animals
Epigenetics
Molecular Biology
Ecology
Evolution
Behavior and Systematics
fungi
DNA Helicases
Reproductive System
Organisms
Biology and Life Sciences
DNA
Cell Biology
G2-M DNA damage checkpoint
Invertebrates
Germ Cells
Animal Studies
DNA damage
Zoology
Entomology
Developmental Biology
Zdroj: PLoS Genetics
PLoS Genetics, Vol 17, Iss 10, p e1009834 (2021)
ISSN: 1553-7404
DOI: 10.1371/journal.pgen.1009834
Popis: Stem cells have the potential to maintain undifferentiated state and differentiate into specialized cell types. Despite numerous progress has been achieved in understanding stem cell self-renewal and differentiation, many fundamental questions remain unanswered. In this study, we identify dRTEL1, the Drosophila homolog of Regulator of Telomere Elongation Helicase 1, as a novel regulator of male germline stem cells (GSCs). Our genome-wide transcriptome analysis and ChIP-Seq results suggest that dRTEL1 affects a set of candidate genes required for GSC maintenance, likely independent of its role in DNA repair. Furthermore, dRTEL1 prevents DNA damage-induced checkpoint activation in GSCs. Finally, dRTEL1 functions to sustain Stat92E protein levels, the key player in GSC maintenance. Together, our findings reveal an intrinsic role of the DNA helicase dRTEL1 in maintaining male GSC and provide insight into the function of dRTEL1.
Author summary Adult tissue homeostasis is maintained by its resident tissue-specific stem cells. The maintenance of stem cells depends on niche activity (the so-called tissue-specific microenvironment) as well as stem cell intrinsic factors. In a genetic screen, we identify dTREL1 as an intrinsic factor required for the maintenance of Drosophila male germline stem cells (GSCs). Loss of dRTEL1 activity leads to premature loss of male GSCs. Our study shows that dRTEL1 likely functions through multiple downstream genes to promote GSC maintenance. Further results indicate that dRTEL1 also prevents activation of the DNA damage response pathway in GSCs for its maintenance. These factors likely converge on the regulation of Stat92E, a well-studied intrinsic factor for GSC maintenance.
Databáze: OpenAIRE
Externí odkaz: