Molecular analysis of Brazilian strains of bovine coronavirus (BCoV) reveals a deletion within the hypervariable region of the S1 subunit of the spike glycoprotein also found in human coronavirus OC43
| Autor: | Leonardo José Richtzenhain, Laura Y. B. Villarreal, F. Gregori, C. A. R. Rosales, José Antonio Jerez, Paulo Eduardo Brandão |
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| Rok vydání: | 2006 |
| Předmět: |
Feline Infectious Peritonitis Virus
Molecular Sequence Data Cattle Diseases Genome Viral medicine.disease_cause Article Protein Structure Secondary Coronavirus OC43 Human Feces Viral Envelope Proteins BOVINOS Nidovirales Sequence Homology Nucleic Acid Virology medicine Animals Cluster Analysis Humans Coronaviridae Human coronavirus OC43 Amino Acid Sequence Gene Sequence Deletion Coronavirus Bovine coronavirus Coronavirus Bovine Genetics Membrane Glycoproteins Sequence Homology Amino Acid biology Spike Protein Reverse Transcriptase Polymerase Chain Reaction General Medicine biology.organism_classification Spike Glycoprotein Hypervariable region Hypervariable Region GenBank Spike Glycoprotein Coronavirus RNA Viral Cattle Coronavirus Infections Brazilian Strain Brazil |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Archives of Virology |
| Popis: | Summary. Bovine coronavirus (BCoV) causes enteric and respiratory dis- orders in calves and dysentery in cows. In this study, 51 stool samples of calves from 10 Brazilian dairy farms were analysed by an RT-PCR that amplifies a 488-bp fragment of the hypervariable region of the spike glycoprotein gene. Maximum parsimony genealogy with a heuristic algorithm using sequences from 15 field strains studied here and 10 sequences from GenBank and bredavirus as an outgroup virus showed the existence of two major clusters (1 and 2) in this viral species, the Brazilian strains segregating in both of them. The mean nucleotide identity between the 15 Brazilian strains was 98.34%, with a mean amino acid similarity of 98%. Strains from cluster 2 showed a deletion of 6 amino acids inside domain II of the spike protein that was also found in human coronavirus strain OC43, supporting the recent proposal of a zoonotic spill- over of BCoV. These results contribute to the molecular characterization of BCoV, to the prediction of the efficiency of immunogens, and to the definition of molecular markers useful for epidemiologic surveys on coronavirus-caused diseases. |
| Databáze: | OpenAIRE |
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