Cloning, sequencing and expression of the nhaA and nhaR genes from Salmonella entiritidis
| Autor: | Etana Padan, S. Sela, Herve Bercovier, O. Carmel, Shimon Schuldiner, E Pinner |
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| Rok vydání: | 1992 |
| Předmět: |
Sodium-Hydrogen Exchangers
Protein Conformation Sequence analysis Antiporter Molecular Sequence Data Lithium Biology Molecular cloning medicine.disease_cause Biochemistry Microbiology Conserved sequence Sequence Homology Nucleic Acid Genes Regulator Escherichia coli Genetics medicine Amino Acid Sequence Cloning Molecular Molecular Biology Base Sequence Genetic Complementation Test Sodium Nucleic acid sequence General Medicine Hydrogen-Ion Concentration Molecular biology Complementation Salmonella enteritidis Genes Bacterial Carrier Proteins Hybrid plasmid |
| Zdroj: | Archives of Microbiology. 157:323-328 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/bf00248676 |
| Popis: | Na+/H+ antiporter activity is wide-spread and plays essential physiological roles. We found that several Enterobacteriaceae share conserved sequences with nhaA, the gene coding for an E. coli antiporter. A delta nhaA strain, which is sensitive to Na+ and Li+, was used to clone by complementation a DNA fragment from Salmonella enteritidis which confers resistance to the ions. The cloned fragment increased Na+/H+ antiport activity in membranes isolated from strains carrying the respective hybrid plasmid. DNA sequence analysis of the insert revealed two open reading frames. Both encode putative polypeptides which are closely homologous to the nhaA and nhaR gene products from Escherichia coli. The antiporter activity displays properties very similar to that of the E. coli NhaA, namely, it is activated by alkaline pH and recognizes Li+ with high affinity. |
| Databáze: | OpenAIRE |
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