Coculture with a human granulosa cell line enhanced the development of murine preimplantation embryos via SCF/c-kit system
| Autor: | Tasuku Harada, Naoki Terakawa, Fuminori Taniguchi, Imari Deura, Masahiro Mitsunari, Makoto Nara |
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| Rok vydání: | 2004 |
| Předmět: |
endocrine system
animal structures Cell division endocrine system diseases Granulosa cell Embryonic Development Stem cell factor Biology Article Paracrine signalling Mice Cell Line Tumor Genetics medicine Animals Humans Blastocyst Genetics (clinical) Ovarian Neoplasms Stem Cell Factor Granulosa Cells urogenital system Reverse Transcriptase Polymerase Chain Reaction Embryogenesis Obstetrics and Gynecology Embryo General Medicine Molecular biology Coculture Techniques Cell biology medicine.anatomical_structure Reproductive Medicine Gene Expression Regulation Cell culture embryonic structures Female Cell Division Developmental Biology |
| Zdroj: | Journal of assisted reproduction and genetics. 21(6) |
| ISSN: | 1058-0468 |
| Popis: | Purpose : To evaluate the effect on the coculture of murine embryos with a human ovarian granulosa tumor derived cell line (KGN cells). Methods : We observed microscopically the growth of murine preimplantation embryos in the coculture system with KGN cells or in the presence with exogenous stem cell factor (SCF). The reverse transcriptase–polymerase chain reaction (RT-PCR) method was used to analyze the gene expression of SCF in KGN cells cocultured with murine embryos. Results : The coculture system with KGN cells significantly increased the rate of embryo development to late blastocyst and to hatching stage. We also found that coculture with murine embryos enhanced the gene expression of SCF in KGN cells. Adding human recombinant SCF to the medium significantly enhanced embryo development to late blastocyst and hatching stage. Conclusions : KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine system. |
| Databáze: | OpenAIRE |
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