A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells
| Autor: | Yasuhiko Aiki, Madoka Shimizu, Masaki Warashina, Masakazu Fukuda, Makoto Asashima, Susumu Honda, Keiko Hiemori, Yasuko Onuma, Jun Hirabayashi, Hiroaki Tateno, Yuzuru Ito, Kumiko Higuchi |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Pluripotent Stem Cells
Glycosylation Burkholderia cenocepacia Cellular differentiation Sialoglycoproteins Cell Protein Array Analysis Biotin Biology Ligands Article Flow cytometry chemistry.chemical_compound Lectins medicine Humans Biotinylation Induced pluripotent stem cell Cells Cultured Multidisciplinary medicine.diagnostic_test HEK 293 cells Cell Differentiation Flow Cytometry Molecular biology Coculture Techniques Cell biology medicine.anatomical_structure Cell Transformation Neoplastic HEK293 Cells Podocalyxin chemistry Cell culture |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | While human pluripotent stem cells are attractive sources for cell-replacement therapies, a major concern remains regarding their tumorigenic potential. Thus, safety assessment of human pluripotent stem cell-based products in terms of tumorigenicity is critical. Previously we have identified a pluripotent stem cell-specific lectin probe rBC2LCN recognizing hyperglycosylated podocalyxin as a cell surface ligand. Here we demonstrate that hyperglycosylated podocalyxin is secreted from human pluripotent stem cells into cell culture supernatants. We establish a sandwich assay system, named the GlycoStem test, targeting the soluble hyperglycosylated podocalyxin using rBC2LCN. The GlycoStem test is sufficiently sensitive and quantitative to detect residual human pluripotent stem cells. This work provides a proof of concept for the noninvasive and quantitative detection of tumorigenic human pluripotent stem cells using cell culture supernatants. The developed method should increase the safety of human pluripotent stem cell-based cell therapies. |
| Databáze: | OpenAIRE |
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