Epigenetic regulation of the ELOVL6 gene is associated with a major QTL effect on fatty acid composition in pigs

Autor: Maria Ballester, Josep María Folch, J.A.P. Marchesi, Manuel Revilla, Joaquim Corominas, Anna Puig-Oliveras, Jordi Estellé, Estefania Alves
Přispěvatelé: Ministerio de Economía y Competitividad (España), Ministerio de Educación y Ciencia (España), Universidad Autónoma de Barcelona, Generalitat de Catalunya, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Producció Animal, Genètica i Millora Animal, Ministerio de Economia y Competitividad AGL2011-29821-C02 Spanish Ministerio de Educacion BES-2009-081223 Generalitat de Catalunya ECO/1639/2013 Universitat Autonoma de Barcelona 458-01-1/2011 CNPq scholarship from the Brazilian Science Without Borders Program, European Project: 609398,EC:FP7:PEOPLE,FP7-PEOPLE-2013-COFUND,AGREENSKILLSPLUS(2014)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Swine
[SDV]Life Sciences [q-bio]
Sus scrofa
Adipose tissue
UP-REGULATION
Linkage Disequilibrium
Epigenesis
Genetic
chemistry.chemical_compound
CHROMATIN IMMUNOPRECIPITATION
Palmitoleic acid
Genetics(clinical)
RNA-SEQ
IN-VIVO
Genetics
0303 health sciences
030302 biochemistry & molecular biology
Fatty Acids
METHYLATION
General Medicine
3. Good health
ESTROGEN RESPONSE ELEMENTS
ADIPOSE-TISSUE
Adipose Tissue
EXPRESSION
663/664
Quantitative Trait Loci
Single-nucleotide polymorphism
Biology
Quantitative trait locus
Polymorphism
Single Nucleotide
03 medical and health sciences
LIPID-METABOLISM
SNP
Animals
Allele
Sensory disorders Radboud Institute for Molecular Life Sciences [Radboudumc 12]
Gene
INTEGRATIVE GENOMICS VIEWER
Ecology
Evolution
Behavior and Systematics
Alleles
Crosses
Genetic
030304 developmental biology
Research
Promoter
DNA
Molecular biology
Chromosomes
Mammalian
chemistry
Mutation
Animal Science and Zoology
Zdroj: Genetics, Selection, Evolution, 47, 20
Genetics Selection Evolution (47), . (2015)
Genetics, Selection, Evolution : GSE
Digital.CSIC. Repositorio Institucional del CSIC
instname
Repositorio de Resultados de Investigación del INIA
INIA: Repositorio de Resultados de Investigación del INIA
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
Genetics Selection Evolution
Genetics Selection Evolution, 2015, 47 (1), pp.20. ⟨10.1186/s12711-015-0111-y⟩
Genetics, Selection, Evolution, 47, pp. 20
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)
Genetics Selection Evolution, BioMed Central, 2015, 47 (1), pp.20. ⟨10.1186/s12711-015-0111-y⟩
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA
ISSN: 0999-193X
1297-9686
DOI: 10.1186/s12711-015-0111-y⟩
Popis: [Background]: In previous studies on an Iberian x Landrace cross, we have provided evidence that supported the porcine ELOVL6 gene as the major causative gene of the QTL on pig chromosome 8 for palmitic and palmitoleic acid contents in muscle and backfat. The single nucleotide polymorphism (SNP) ELOVL6:c.-533C > T located in the promoter region of ELOVL6 was found to be highly associated with ELOVL6 expression and, accordingly, with the percentages of palmitic and palmitoleic acids in longissimus dorsi and adipose tissue. The main goal of the current work was to further study the role of ELOVL6 on these traits by analyzing the regulation of the expression of ELOVL6 and the implication of ELOVL6 polymorphisms on meat quality traits in pigs.
[Results]: High-throughput sequencing of BAC clones that contain the porcine ELOVL6 gene coupled to RNAseq data re-analysis showed that two isoforms of this gene are expressed in liver and adipose tissue and that they differ in number of exons and 3’UTR length. Although several SNPs in the 3’UTR of ELOVL6 were associated with palmitic and palmitoleic acid contents, this association was lower than that previously observed with SNP ELOVL6:c.-533C > T. This SNP is in full linkage disequilibrium with SNP ELOVL6:c.-394G > A that was identified in the binding site for estrogen receptor alpha (ERα). Interestingly, the ELOVL6:c.-394G allele is associated with an increase in methylation levels of the ELOVL6 promoter and with a decrease of ELOVL6 expression. Therefore, ERα is clearly a good candidate to explain the regulation of ELOVL6 expression through dynamic epigenetic changes in the binding site of known regulators of ELOVL6 gene, such as SREBF1 and SP1.
[Conclusions]: Our results strongly suggest the ELOVL6:c.-394G > A polymorphism as the causal mutation for the QTL on pig chromosome 8 that affects fatty acid composition in pigs.
This work was funded by the Ministerio de Economía y Competitividad project AGL2011-29821-C02. J Corominas was funded by a FPI PhD grant from the Spanish Ministerio de Educación (BES-2009-081223), M Revilla was funded by a Formació i Contractació de Personal Investigador Novell (FI-DGR) PhD grant from Generalitat de Catalunya (ECO/1639/2013), A Puig-Oliveras was funded by a PIF PhD grant from the Universitat Autónoma de Barcelona (458-01-1/2011) and JAP Marchesi was funded by a CNPq scholarship from the Brazilian Science Without Borders Program.
Databáze: OpenAIRE
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