Pseudophosphatases: methods of analysis and physiological functions
| Autor: | Dmitri Kharitidi, Sanaz Manteghi, Arnim Pause |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Binding Sites Cellular homeostasis Protein tyrosine phosphatase Biology General Biochemistry Genetics and Molecular Biology Cell biology Substrate Specificity Enzyme Human disease chemistry Humans Signal transduction Protein Tyrosine Phosphatases Phosphotyrosine-binding domain Molecular Biology Sequence Alignment Enzyme Assays |
| Zdroj: | Methods (San Diego, Calif.). 65(2) |
| ISSN: | 1095-9130 |
| Popis: | Protein tyrosine phosphatases (PTPs) are key enzymes in the regulation of cellular homeostasis and signaling pathways. Strikingly, not all PTPs bear enzymatic activity. A considerable fraction of PTPs are enzymatically inactive and are known as pseudophosphatases. Despite the lack of activity they execute pivotal roles in development, cell biology and human disease. The present review is focused on the methods used to identify pseudophosphatases, their targets, and physiological roles. We present a strategy for detailed enzymatic analysis of inactive PTPs, regulation of inactive PTP domains and identification of binding partners. Furthermore, we provide a detailed overview of human pseudophosphatases and discuss their regulation of cellular processes and functions in human pathologies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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