Proteome and Transcriptome Analysis of the Antioxidant Mechanism in Chicken Regulated by Eucalyptus Leaf Polyphenols Extract
Autor: | Yunjiao Chen, Ze-Qi He, Xiaoying Zhang, Yong Cao, Wei Li, Jian-Jun Zuo |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Aging Antioxidant Article Subject Proteome medicine.medical_treatment Biology Biochemistry Antioxidants Transcriptome 03 medical and health sciences chemistry.chemical_compound In vivo medicine Animals RNA Messenger RNA-Seq Food science chemistry.chemical_classification Eucalyptus QH573-671 Plant Extracts Gene Expression Profiling Muscles Glutathione peroxidase 0402 animal and dairy science Polyphenols Molecular Sequence Annotation 04 agricultural and veterinary sciences Cell Biology General Medicine Glutathione Peroxisome 040201 dairy & animal science Metabolic pathway Gene Ontology 030104 developmental biology Gene Expression Regulation chemistry Polyphenol Dietary Supplements Cytology Chickens Research Article |
Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2020 (2020) Oxidative Medicine and Cellular Longevity |
ISSN: | 1942-0994 1942-0900 |
Popis: | Eucalyptus leaf polyphenols extract (EPE) has been proved to have various bioactivities, but few reports focus on its antioxidant mechanism in vivo. The purpose of this study was to elucidate the effect and mechanism of EPE dietary supplements on antioxidant capacity in chicken. A total of 216 chickens were randomly selected for a 40-day experiment. Four treatment groups received diets including the control diet only, the control diet + low EPE (0.6 g/kg), the control diet + moderate EPE (0.9 g/kg), and the control diet + high EPE (1.2 g/kg). Compared with control group, the glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content in the breast muscle of the moderate EPE treatment group was significantly higher (p<0.05), while the malonaldehyde (MDA) content in the moderate EPE group was reduced (p<0.05). Moreover, proteomic and transcriptomic analyses of the breast muscle revealed that glutathione metabolism and the peroxisome were the two crucial metabolic pathways responsible for increased antioxidant capacity of the muscle. Accordingly, nine candidate genes and two candidate proteins were identified related to improved antioxidant status induced by EPE supplements. This research provides new insights into the molecular mechanism of antioxidant capacity in chickens treated with EPE dietary supplements. |
Databáze: | OpenAIRE |
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