Irradiation with narrowband-ultraviolet B suppresses phorbol ester-induced up-regulation of H1receptor mRNA in HeLa cells

Autor:	Yoshiaki Kitamura, Noriaki Takeda, Toshio Matsushita, Tatsuya Fujii, Hiroyuki Mizuguchi, Hiroyuki Fukui, Mika Kitayama, Yoshiaki Kubo, Takashi Mukai, Akira Fujioka, Kentaro Okamoto, Nobuo Kubo
Rok vydání:	2016
Předmět:	0301 basic medicine Histamine H1 receptor Biology Ultraviolet therapy HeLa 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Phorbol Esters Gene expression Humans Receptors Histamine H1 skin and connective tissue diseases integumentary system Epithelial Cells General Medicine biology.organism_classification Rhinitis Allergic Molecular biology 030104 developmental biology Otorhinolaryngology Biochemistry UVB-induced apoptosis Apoptosis DNA fragmentation Ultraviolet Therapy 030217 neurology & neurosurgery HeLa Cells
Zdroj:	Acta Oto-Laryngologica. 136:409-413
ISSN:	1651-2251 0001-6489
DOI:	10.3109/00016489.2015.1129555
Popis:	Conclusion These findings suggest that low dose irradiation with 310 nm NB-UVB specifically suppressed the up-regulation of H1R gene expression without inducing apoptosis and that UVB of shorter or longer wavelength than 310 nm NB-UVB had no such effects. Objective To develop a narrowband-ultraviolet B(NB-UVB) phototherapy for allergic rhinitis, this study investigated the effects of irradiation with NB-UVB at wavelength of 310 nm on phorbol-12-myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) mRNA in HeLa cells. Methods The mRNA levels of H1R in HeLa cells were measured using real-time RT-PCR. Apoptosis were evaluated with DNA fragmentation assay. Results PMA induced a significant increase in H1R mRNA expression in HeLa cells. Irradiation with 305 nm UVB and 310 nm NB-UVB, but not with 315 nm UVB at doses of 200 and 300 mJ/cm(2) significantly suppressed PMA-induced up-regulation of H1R mRNA. At a dose of 200 mJ/cm(2), irradiation with 305 nm UVB, but not with 310 nm NB-UVB, induced apoptosis, although exposure of the cells to both 305 and 310 nm UVB induced apoptosis at a dose of 300 mJ/cm(2) after PMA treatment in HeLa cells. Conversely, irradiation with 315 nm UVB at doses of 200 and 300 mJ/cm(2) did not induce apoptosis.
Databáze:	OpenAIRE
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