A specific and bioactive polysaccharide marker for Cordyceps
| Autor: | Karl Wah Keung Tsim, Hoi-Yan Wu, Man Liu, Li-Feng Li, Tin-Long Wong, Grace Wing-Chiu But, Ji-Xia Zhang, Hui Cao, Hau-Yee Fung, Quan-Wei Zhang, Pema-Tenzin Puno, Su-Ping Bai, Xin Wen, Zhongzhen Zhao, Hui-Yuan Cheng, Pang-Chui Shaw, Quan-Bin Han, Han-Dong Sun, Miao-Miao Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
Screening test Cell Survival Food Contamination 02 engineering and technology Moths 010402 general chemistry Polysaccharide Nitric Oxide 01 natural sciences Mice Materials Chemistry Animals Immunologic Factors Glucans chemistry.chemical_classification Cordyceps biology Organic Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences RAW 264.7 Cells Biochemistry chemistry Carbohydrate Sequence Genetic marker 0210 nano-technology Biomarkers |
| Zdroj: | Carbohydrate polymers. 269 |
| ISSN: | 1879-1344 |
| Popis: | Cordyceps is one of the most expensive and widely used functional foods. But the authenticity is still a concern due to the lack of appropriate markers. By targeting polysaccharides, this study aimed to develop a specific, and bioactive marker for Cordyceps. Firstly, the results of screening tests of 250 samples by examining both genetic markers and polysaccharide profile showed that a unique polysaccharide fraction (named CCP) was particular to the caterpillar parts. Its potential as a marker was further demonstrated by its ability to induce NO and cytokine production in RAW 264.7 cells. CCP was characterized to be an α-1,4-glucan with a branch at C-6 by the conventional structure analyzing and de novo oligosaccharides sequencing. The content of CCP was closely correlated to the traditional classification criteria. Generally, CCP was a marker that simultaneously enables qualitative and quantitative analysis of Cordyceps. |
| Databáze: | OpenAIRE |
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