In vivo ameliorative effects of methanol leaf extract of Lawsonia inermis Linn on experimental Trypanosoma congolense infection in Wistar rats

Autor: Patricia Ishaku Kobo, Salisu Hashim Shittu, B. Habibu, Mohammed M. Suleiman, Peter Ofemile Yusuf, Mohammed Umar Kawu, Abdullah Mohammad Tauheed
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: International Journal of Veterinary Science and Medicine, Vol 4, Iss 2, Pp 33-40 (2016)
ISSN: 2314-4599
Popis: The aim of this study was to investigate the ameliorative effect of Lawsonia inermis Linn used traditionally against trypanosomosis. Twenty-five adult Wistar rats of both sex were individually infected intraperitoneally (IP) with 106 Trypanosoma congolense per ml of blood. Following establishment of infection, the rats were randomly divided into five groups of 5 rats each. Rats in groups I, II, and III were treated with 125, 250 and 500 mg/kg of the extract, respectively, while rats in groups IV and V were treated with 3.5 mg/kg and 2 ml/kg of diminazene aceturate (DM) once and physiological buffered saline, respectively. All treatments except DM were given orally for 7 days IP. The antitrypanosomal effect of the plant was assessed by observing the level of parasitaemia daily, packed cell volume (PCV) weekly, erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration on day 21. Phytochemical screening of the extract revealed the presence of alkaloids, carbohydrates, triterpenes, steroids, cardiac glycosides, saponins, tannins and flavonoids. The extract significantly (P 0.05) in extract treated groups but significantly higher (P
Databáze: OpenAIRE