Lentivirus-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cells

Autor: Florence Leuba, Odile Ducrey-Rundquist, Shahnaz Abraham, Maciej Wiznerowicz, Valérie Dutoit, Vincent Piguet, Marjorie Pion, Didier Trono, Jean-François Arrighi, Teunis B. Geijtenbeek, Yvette van Kooyk, Eduardo Garcia
Přispěvatelé: Econometrics and Operations Research, Other departments, Molecular cell biology and Immunology
Jazyk: angličtina
Rok vydání: 2004
Předmět:
Zdroj: Arrighi, J-F, Pion, M, Wiznerowicz, M, Geijtenbeek, T B, Garcia, E, Abraham, S, Leuba, F, Dutoit, V, Ducrey-Rundquist, O, van Kooyk, Y, Trono, D & Piguet, V 2004, ' Lentivirus-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cells ', Journal of Virology, vol. 78, no. 20, pp. 10848-55 . https://doi.org/10.1128/JVI.78.20.10848-10855.2004
Journal of Virology
Journal of Virology, 20, 10848-10855. American Society for Microbiology
Arrighi, JF, Pion, M, Wiznerowics, M, Geijtenbeek, T B H, Garcia, E, Abraham, S, Leuba, F, Dutoit, V, Ducrey-Rundquist, O, van Kooijk, Y, Trono, D & Piquet, V 2004, ' Lentiviral-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cells ', Journal of Virology, vol. 20, pp. 10848-10855 . https://doi.org/10.1128/JVI.78.20.10848-10855.2004
Journal of virology, 78(20), 10848-10855. American Society for Microbiology
Arrighi, JF, Pion, M, Wiznerowicz, M, Geijtenbeek, T B H, Garcia, E, Abraham, S, Leuba, F, Dutoit, V, Ducrey-Rundquist, O, van Kooijk, Y, Trono, D & Piguet, V 2004, ' Lentivirus-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cells. ', Journal of Virology, vol. 78, no. 20, pp. 10848-55 . https://doi.org/10.1128/JVI.78.20.10848-10855.2004
Journal of Virology, 78(20), 10848-55. American Society for Microbiology
ISSN: 0022-538X
DOI: 10.1128/JVI.78.20.10848-10855.2004
Popis: In the early events of human immunodeficiency virus type 1 (HIV-1) infection, immature dendritic cells (DCs) expressing the DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) receptor capture small amounts of HIV-1 on mucosal surfaces and spread viral infection to CD4 + T cells in lymph nodes (22, 34, 45). RNA interference has emerged as a powerful tool to gain insight into gene function. For this purpose, lentiviral vectors that express short hairpin RNA (shRNA) for the delivery of small interfering RNA (siRNA) into mammalian cells represent a powerful tool to achieve stable gene silencing. In order to interfere with DC-SIGN function, we developed shRNA-expressing lentiviral vectors capable of conditionally suppressing DC-SIGN expression. Selectivity of inhibition of human DC-SIGN and L-SIGN and chimpanzee and rhesus macaque DC-SIGN was obtained by using distinct siRNAs. Suppression of DC-SIGN expression inhibited the attachment of the gp120 envelope glycoprotein of HIV-1 to DC-SIGN transfectants, as well as transfer of HIV-1 to target cells in trans . Furthermore, shRNA-expressing lentiviral vectors were capable of efficiently suppressing DC-SIGN expression in primary human DCs. DC-SIGN-negative DCs were unable to enhance transfer of HIV-1 infectivity to T cells in trans , demonstrating an essential role for the DC-SIGN receptor in transferring infectious viral particles from DCs to T cells. The present system should have broad applications for studying the function of DC-SIGN in the pathogenesis of HIV as well as other pathogens also recognized by this receptor.
Databáze: OpenAIRE