Escherichia coli Maltose-Binding Protein Induces M1 Polarity of RAW264.7 Macrophage Cells via a TLR2- and TLR4-Dependent Manner
| Autor: | Fang Wang, Wan Wang, Weihua Ni, Guomu Liu, Hongyan Yuan, Guixiang Tai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Cell Survival
MAP Kinase Signaling System toll-like receptor 4 Nitric Oxide Synthase Type II Nitric Oxide p38 Mitogen-Activated Protein Kinases Catalysis Article lcsh:Chemistry Inorganic Chemistry Maltose-binding protein Mice Immune system toll-like receptor 2 Animals Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Maltodextrin transport biology Escherichia coli Proteins Macrophages Organic Chemistry Histocompatibility Antigens Class I NF-kappa B Cell Polarity General Medicine Molecular biology Computer Science Applications Up-Regulation Nitric oxide synthase TLR2 RAW 264.7 Cells lcsh:Biology (General) lcsh:QD1-999 maltose-binding protein Periplasmic Binding Proteins biology.protein classically activated macrophages Phosphorylation Cytokines Pinocytosis Inflammation Mediators Cell activation CD80 Biomarkers |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 16, Iss 5, Pp 9896-9909 (2015) Volume 16 Issue 5 Pages 9896-9909 |
| ISSN: | 1422-0067 |
| Popis: | Maltose-binding protein (MBP) is a critical player of the maltose/maltodextrin transport system in Escherichia coli. Our previous studies have revealed that MBP nonspecifically induces T helper type 1 (Th1) cell activation and activates peritoneal macrophages obtained from mouse. In the present study, we reported a direct stimulatory effect of MBP on RAW264.7 cells, a murine macrophage cell line. When stimulated with MBP, the production of nitric oxide (NO), IL-1β, IL-6 and IL-12p70, and the expressions of CD80, MHC class II and inducible nitric oxide synthase (iNOS) were all increased in RAW264.7 cells, indicating the activation and polarization of RAW264.7 cells into M1 macrophages induced by MBP. Further study showed that MBP stimulation upregulated the expression of TLR2 and TLR4 on RAW264.7 cells, which was accompanied by subsequent phosphorylation of IκB-α and p38 MAPK. Pretreatment with anti-TLR2 or anti-TLR4 antibodies largely inhibited the phosphorylation of IκB-α and p38 MAPK, and greatly reduced MBP-induced NO and IL-12p70 production, suggesting that the MBP-induced macrophage activation and polarization were mediated by TLR2 and TLR4 signaling pathways. The observed results were independent of lipopolysaccharide contamination. Our study provides a new insight into a mechanism by which MBP enhances immune responses and warrants the potential application of MBP as an immune adjuvant in immune therapies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|