| Autor: |
Pauline Nibbe, Johannes Schleusener, Silas Siebert, Richard Borgart, Doreen Brandt, Ronja Westphalen, Nadine Schüler, Bastian Berger, Eva M.J. Peters, Martina C. Meinke, Silke B. Lohan |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Free Radical Biology and Medicine. 194:230-244 |
| ISSN: |
0891-5849 |
| DOI: |
10.1016/j.freeradbiomed.2022.11.034 |
| Popis: |
Oxidative stress as a driver of disease is reinforcing the trend towards supplementation with antioxidants. While antioxidants positively influence the redox status when applied at physiological doses, higher concentrations may have pro-oxidative effects. Precise assessment methods for testing the supply of antioxidants are lacking. Using in-situ-irradiation as stressor and electron paramagnetic resonance (EPR) spectroscopy as readout system for formed radicals, a stress response assessment method was developed, using protein solutions and plasma samples from transfusion medicine. The method was validated in a double-blind placebo-controlled in vivo cross-over pilot study in blood plasma samples of individuals before and after vitamin C supplementation. Reference measurements were performed for the exogenous antioxidants β-carotene and vitamin C, and glutathione as an endogenous representative. Malondialdehyde was studied for oxidative stress indication. Protein solutions without antioxidants showed a linear increase in radical concentration during irradiation. The in-vitro-addition of vitamin C or plasma samples from subjects displayed two slopes (m |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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