Imaging lung function in mice using SPECT/CT and per-voxel analysis
| Autor: | N. Renée Labiris, Rod G. Rhem, Iris Q. Wang, Cory A. J. R. McCurry, Brian N. Jobse |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Lung Diseases
medicine.medical_specialty Anatomy and Physiology Mouse Radionuclide imaging Respiratory System lcsh:Medicine Respiratory physiology Single-photon emission computed tomography computer.software_genre Multimodal Imaging Ventilation/perfusion ratio Diagnostic Radiology Pulmonary function testing Mice Model Organisms Computed Tomography Voxel medicine Animals Respiratory Physiology lcsh:Science Lung Biology Mice Inbred BALB C SPECT imaging Multidisciplinary medicine.diagnostic_test business.industry lcsh:R Animal Models medicine.anatomical_structure Positron-Emission Tomography Chronic Disease Nuclear medicine Breathing Medicine Female lcsh:Q Radiology Pulmonary Ventilation Tomography X-Ray Computed business Perfusion computer Research Article |
| Zdroj: | PLoS ONE, Vol 7, Iss 8, p e42187 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Chronic lung disease is a major worldwide health concern but better tools are required to understand the underlying pathologies. Ventilation/perfusion (V/Q) single photon emission computed tomography (SPECT) with per-voxel analysis allows for non-invasive measurement of regional lung function. A clinically adapted V/Q methodology was used in healthy mice to investigate V/Q relationships. Twelve week-old mice were imaged to describe normal lung function while 36 week-old mice were imaged to determine how age affects V/Q. Mice were ventilated with Technegas™ and injected with (99m)Tc-macroaggregated albumin to trace ventilation and perfusion, respectively. For both processes, SPECT and CT images were acquired, co-registered, and quantitatively analyzed. On a per-voxel basis, ventilation and perfusion were moderately correlated (R = 0.58±0.03) in 12 week old animals and a mean log(V/Q) ratio of -0.07±0.01 and standard deviation of 0.36±0.02 were found, defining the extent of V/Q matching. In contrast, 36 week old animals had significantly increased levels of V/Q mismatching throughout the periphery of the lung. Measures of V/Q were consistent across healthy animals and differences were observed with age demonstrating the capability of this technique in quantifying lung function. Per-voxel analysis and the ability to non-invasively assess lung function will aid in the investigation of chronic lung disease models and drug efficacy studies. |
| Databáze: | OpenAIRE |
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