New fluorogenic probes for neutral and alkaline ceramidases
| Autor: | Cungui Mao, Núria Bielsa, Josefina Casas, Laura Bassas, Gemma Fabriàs, Antonio Delgado, Mireia Casasampere, José Luis Abad, Ruijuan Xu, Daniel Riba |
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| Přispěvatelé: | Fabriàs, Gemma, Fabriàs, Gemma [0000-0001-7162-3772] |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Síntesi orgànica Magnetic Resonance Spectroscopy Ceramidases Organic synthesis substrate QD415-436 Fluorogenic 030204 cardiovascular system & hematology Alkaline Ceramidase Ceramides Biochemistry Cell Line 03 medical and health sciences chemistry.chemical_compound umbelliferone 0302 clinical medicine Endocrinology Amide Microsomes Methods Humans Chemical synthesis Umbelliferones Cancer Sphingolipids Neutral Ceramidase Molecular Structure Intracellular Signaling Peptides and Proteins Substrate (chemistry) RNA-Binding Proteins Lipase Cell Biology Ceramidase Lipids Combinatorial chemistry Acid Ceramidase 030104 developmental biology chemistry Substrate HT29 Cells Nervonic acid Esfingolípids |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona J Lipid Res Journal of Lipid Research, Vol 60, Iss 6, Pp 1174-1181 (2019) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | New fluorogenic ceramidase substrates derived from the N acyl modification of our previously reported probes (RBM14) are reported. While none of the new probes were superior to the known RBM14C12 as acid ceramidase substrates, the corresponding nervonic acid amide (RBM14C24:1) is an efficient and selective substrate for the recombinant human neutral ceramidase, both in cell lysates and in intact cells. A second generation of substrates, incorporating the natural 2 (N acylamino) 1,3 diol 4 ene framework (compounds RBM15) is also reported. Among them, the corresponding fatty acyl amides with an unsaturated N acyl chain can be used as substrates to determine ACER1 and ACER2 activities. In particular, compound RBM15C18:1 has emerged as the best fluorogenic probe reported so far to measure ACER1 and ACER2 activities in a 96 well plate format. |
| Databáze: | OpenAIRE |
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