Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet
| Autor: | Hiroshi Yaginuma, Taku Tsunekawa, Keigo Taki, Takeshi Onoue, Runan Sun, Yoshihiro Ito, Shintaro Iwama, Tomoko Kobayashi, Ryoichi Banno, Hiroshi Arima, Daisuke Sakano, Daisuke Hagiwara, Hiroshi Takagi, Akira Mizoguchi, Shoen Kume, Tomonori Hirose, Hidetaka Suga, Mariko Sugiyama |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine Physiology Gene Expression White adipose tissue Biochemistry Chloride Mice Endocrinology 0302 clinical medicine Insulin-Secreting Cells Insulin Secretion Medicine and Health Sciences Insulin Glucose tolerance test Multidisciplinary geography.geographical_feature_category medicine.diagnostic_test Chemistry digestive oral and skin physiology food and beverages Islet Body Fluids Blood medicine.anatomical_structure Adipose Tissue Physiological Parameters Connective Tissue Medicine lipids (amino acids peptides and proteins) Anatomy Pancreas hormones hormone substitutes and hormone antagonists Research Article medicine.drug medicine.medical_specialty Adipose Tissue White Science Carbohydrate metabolism Diet High-Fat Blood Plasma 03 medical and health sciences Dietary Sodium Internal medicine Glucose Intolerance Genetics medicine Animals Sodium Chloride Dietary Nutrition Diabetic Endocrinology geography Body Weight Insulin tolerance test Biology and Life Sciences nutritional and metabolic diseases Glucose Tolerance Test Hormones Diet Biological Tissue Metabolism 030104 developmental biology Food Insulin Resistance Energy Metabolism 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 16, Iss 3, p e0248065 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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