Non‐biofilm‐forming commensalStaphylococcus epidermidisisolates produce biofilm in the presence of trypsin
| Autor: | Maria de Jesus de Haro-Cruz, Sergio Martínez-García, Gabriel Betanzos-Cabrera, Janet Jan-Roblero, Sonia Mayra Pérez-Tapia, Silvestre Ortega-Peña, Ma Guadalupe Aguilera-Arreola, Juan C. Cancino-Diaz, Mario E. Cancino-Diaz, Sandra Rodríguez-Martínez, María Dolores Alcántar-Curiel |
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| Rok vydání: | 2019 |
| Předmět: |
Prosthesis-Related Infections
Eye Diseases Genotype Prosthetic joint Mrna expression lcsh:QR1-502 Microbiology lcsh:Microbiology Bacterial Proteins Staphylococcus epidermidis Osteoarthritis medicine Humans non‐biofilm‐forming Skin biology Gene Expression Profiling Ocular Infections Significant difference Biofilm Original Articles Staphylococcal Infections biochemical phenomena metabolism and nutrition Trypsin biology.organism_classification Healthy Volunteers trypsin Biofilms protease‐independent biofilm Original Article total biofilm medicine.drug |
| Zdroj: | MicrobiologyOpen, Vol 8, Iss 10, Pp n/a-n/a (2019) MicrobiologyOpen |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.906 |
| Popis: | Epidemiological studies comparing clinical and commensal Staphylococcus epidermidis isolates suggest that biofilm formation is a discriminant biomarker. A study showed that four non‐biofilm‐forming clinical S. epidermidis isolates could form an induced biofilm by trypsin treatment, suggesting that S. epidermidis can form biofilms in a protease‐independent way and in a trypsin‐induced way. In this study, the trypsin capacity to induce biofilm formation was evaluated in non‐biofilm‐forming S. epidermidis isolates (n = 133) in order to support this mechanism and to establish the importance of total biofilms (meaning the sum of protease‐independent biofilm and trypsin‐induced biofilm). Staphylococcus epidermidis isolates from ocular infections (OI; n = 24), prosthetic joint infections (PJI; n = 64), and healthy skin (HS‐1; n = 100) were screened for protease‐independent biofilm formation according to Christensen's method. The result was that there are significant differences (p Epidemiological studies comparing clinical and commensal Staphylococcus epidermidis isolates suggest that biofilm formation is a discriminant biomarker. In this study, the capacity of trypsin to induce biofilm formation was evaluated in non‐biofilm‐forming S. epidermidis isolates. Trypsin is capable to induce biofilm production in non‐biofilm‐forming commensal S. epidermidis isolates with the icaA −/aap+ genotype |
| Databáze: | OpenAIRE |
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