Ribosomal DNA transcription in prefrontal pyramidal neurons is decreased in suicide
| Autor: | Krzysztof Rębała, Zbigniew Jankowski, Marta Krzyżanowska, Dorota Pieśniak, Michał Kaliszan, Karol Karnecki, Monika Kosmowska, Johann Steiner, Marek Wiergowski, Tomasz Gos, Katharina Braun, Ralf Brisch, Joanna Chociej |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty AgNOR staining Prefrontal Cortex Biology DNA Ribosomal Gyrus Cinguli Pathogenesis 03 medical and health sciences 0302 clinical medicine Sex Factors Transcription (biology) Internal medicine Suicide Completed medicine Protein biosynthesis Humans Pharmacology (medical) Prefrontal cortex Ribosomal DNA Biological Psychiatry Anterior cingulate cortex Transcriptional activity Original Paper Staining and Labeling Pyramidal Cells General Medicine Middle Aged 030227 psychiatry Psychiatry and Mental health Suicide medicine.anatomical_structure Endocrinology Postmortem Orbitofrontal cortex Female 030217 neurology & neurosurgery |
| Zdroj: | European Archives of Psychiatry and Clinical Neuroscience |
| ISSN: | 1433-8491 |
| Popis: | Prefrontal cortical regions, which are crucial for the regulation of emotionally influenced behaviour, play most probably a dominant role in the pathogenesis of suicide. The study was carried out on paraffin-embedded brain tissue blocks containing specimens from the anterior cingulate cortex (dorsal and ventral parts), the orbitofrontal cortex, and the dorsolateral cortex obtained from 23 suicide completers (predominantly violent) with unknown psychiatric diagnosis and 25 non-suicidal controls. The transcriptional activity of ribosomal DNA (rDNA) as a surrogate marker of protein biosynthesis was evaluated separately in layers III and V pyramidal neurons in regions of interest (ROIs) mentioned above by the AgNOR silver staining method bilaterally. The overall statistical analysis revealed a decrease of AgNOR area suggestive of attenuated rDNA activity in suicide victims versus controls, particularly in male subjects. Further ROI-specific post-hoc analyses revealed decreases of the median AgNOR area in suicides compared to non-suicides in all 16 ROIs. However, this effect was only significant in the layer V pyramidal neurons of the right ventral anterior cingulate cortex. Our findings suggest that decreased rDNA transcription in prefrontal pyramidal neurons plays possibly an important role in suicide pathogenesis. Electronic supplementary material The online version of this article (10.1007/s00406-019-00996-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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